Universidade Federal De Pernambuco Centro De Ciências Biológicas Programa De Pós-graduação Em Biotecnologia Industrial

Content Provider	Semantic Scholar
Author	Arrais, Luciana Gomes
Copyright Year	2012
Abstract	The interest in research related to endophytes and their biotechnological properties, increases as the need arises by the search for new bioactive metabolites, produced by these species that can combat multi-resistant microorganisms. Endophytic microorganisms were isolated from healthy leaves of Schinus terebinthifolius Raddi (Aroeira-Red) and they were evaluated for antimicrobial activity against pathogenic microorganisms through a primary test with blocks of agar, and liquid fermentation tests with their extracts. The extracts were analyzed by chemical prospecting and bioautography thin layer. There was obtained 237 fungal isolates, 67 bacterial and 13 actinobacteria. Were tested forty fungi and 13 actinomycetes, of which 8 (20%) and 13 (100%) had inhibitory activity, respectively. We performed liquid fermentation with the fungus (Mycelium stereo F-61) and the actinobacteria (Streptomyces kunmingensis Act-3) which has better inhibition zones. The ethereal fractions (pH 7.0) from the M. stereo F-61, could inhibit S. aureus (20 mm), E. coli (23 mm) and S. aureus methicillin-resistant (MRSA) (23 mm). The fractions of ethyl acetate (pH 7.0) derived from S. kunmingensis Act-3 inhibited S. aureus (29 mm) and S. aureus (MRSA) (20 mm). These extracts showed MIC for S. kunmingensis (NBRC 14463) against S. aureus (62 mg mL-1) and S. aureus (MRSA) (125 mg mL-1), and the M. stereo F-61 against S. aureus (125 mg mL-1) S. aureus (MRSA) (500 mg mL-1) and E. coli (500 mg mL-1). The prospect of chemical extracts of M. stereo F-61 revealed the presence of sugars and phenolic compounds in methanol extracts, and triterpenes, steroids, mono / sesquiterpenes and phenolic compounds in the ether extract. The bioautography found that the active compounds produced by S. kunmingensis Act-3 and the M. stereo F-61 is not similar to those produced by the plant. We conclude that the isolates produce bioactive secondary metabolites with antimicrobial activity, and exhibit potential for the discovery of a new bioactive substance of biotechnological interest.
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Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article