Neurokinins and inflammatory cell iNOS expression in guinea pigs with chronic allergic airway inflammation.

Content Provider	Semantic Scholar
Author	Prado, Carla Máximo Leick-Maldonado, Edna Aparecida Arata, Vanessa Kasahara, David Itiro Martins, Milton A. Tibério, Iolanda De Fátima Lopes Calvo
Copyright Year	2005
Abstract	In the present study we evaluated the role of neurokinins in the modulation of inducible nitric oxide synthase (iNOS) inflammatory cell expression in guinea pigs with chronic allergic airway inflammation. In addition, we studied the acute effects of nitric oxide inhibition on this response. Animals were anesthetized and pretreated with capsaicin (50 mg/kg sc) or vehicle 10 days before receiving aerosolized ovalbumin or normal saline twice weekly for 4 wk. Animals were then anesthetized, mechanically ventilated, given normal saline or N(G)-nitro-l-arginine methyl ester (l-NAME, 50 mg/kg ic), and challenged with ovalbumin. Prechallenge exhaled NO increased in ovalbumin-exposed guinea pigs (P < 0.05 compared with controls), and capsaicin reduced this response (P < 0.001). Compared with animals inhaled with normal saline, ovalbumin-exposed animals presented increases in respiratory system resistance and elastance and numbers of total mononuclear cells and eosinophils, including those expressing iNOS (P < 0.001). Capsaicin reduced all these responses (P < 0.05) except for iNOS expression in eosinophils. Treatment with l-NAME increased postantigen challenge elastance and restored both resistance and elastance previously attenuated by capsaicin treatment. Isolated l-NAME administration also reduced total eosinophils and mononuclear cells, as well as those cells expressing iNOS (P < 0.05 compared with ovalbumin alone). Because l-NAME treatment restored lung mechanical alterations previously attenuated by capsaicin, NO and neurokinins may interact in controlling airway tone. In this experimental model, NO and neurokinins modulate eosinophil and lymphocyte infiltration in the airways.
Starting Page	5752
Ending Page	5755
Page Count	4
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://ajplung.physiology.org/content/ajplung/early/2004/12/03/ajplung.00208.2004.full.pdf
Alternate Webpage(s)	http://ajplung.physiology.org/content/ajplung/288/4/L741.full.pdf
Alternate Webpage(s)	http://ajplung.physiology.org/content/early/2004/12/03/ajplung.00208.2004.full.pdf
PubMed reference number	15579630v1
Volume Number	288
Issue Number	4
Journal	American journal of physiology. Lung cellular and molecular physiology
Language	English
Access Restriction	Open
Subject Keyword	Arginine Capsaicin Cavia Drug Allergy Esters Expiration, function Inspiration function NG-Nitroarginine Methyl Ester Nitric Oxide Synthase Ovalbumin Structure of parenchyma of lung aerosolized liposomal 9-nitro-20 (S) camptothecin eosinophil mg/kg
Content Type	Text
Resource Type	Article