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Morphine-Induced Analgesic Tolerance Effect on Gene Expression of the NMDA Receptor Subunit 1 in Rat Striatum and Prefrontal Cortex
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ahmadi, Shamseddin Rafieenia, Fatemeh Rostamzadeh, Jalal |
| Copyright Year | 2016 |
| Abstract | INTRODUCTION Morphine is a potent analgesic but its continual use results in analgesic tolerance. Mechanisms of this tolerance remain to be clarified. However, changes in the functions of μ-opioid and N-Methyl-D-aspartate (NMDA) receptors have been proposed in morphine tolerance. We examined changes in gene expression of the NMDA receptor subunit 1 (NR1) at mRNA levels in rat striatum and prefrontal cortex (PFC) after induction of morphine tolerance. METHODS Morphine (10 mg/kg, IP) was injected in male Wistar rats for 7 consecutive days (intervention group), but control rats received just normal saline (1 mL/kg, IP). We used a hotplate test of analgesia to assess induction of tolerance to analgesic effects of morphine on days 1 and 8 of injections. Later, two groups of rats were sacrificed one day after 7 days of injections, their whole brains removed, and the striatum and PFC immediately dissected. Then, the NR1 gene expression was examined with a semi-quantitative RT-PCR method. RESULTS The results showed that long-term morphine a administration induces tolerance to analgesic effect of the opioid, as revealed by a significant decrease in morphine-induced analgesia on day 8 compared to day 1 of the injections (P<0.001). The results also showed that the NR1 gene expression at mRNA level in rats tolerant to morphine was significantly increased in the striatum (P<0.01) but decreased in the PFC (P<0.001). CONCLUSION Therefore, changes in the NR1 gene expression in rat striatum and PFC have a region-specific association with morphine-induced analgesic tolerance. |
| Starting Page | 241 |
| Ending Page | 248 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://bcn.iums.ac.ir/article-1-540-fa.pdf |
| Alternate Webpage(s) | http://bcn.iums.ac.ir/browse.php?a_id=540&ftxt=1&sid=1&slc_lang=fa |
| Alternate Webpage(s) | http://bcn.iums.ac.ir/article-1-540-en.pdf%20 |
| Alternate Webpage(s) | http://bcn.iums.ac.ir/browse.php?a_id=540&ftxt=1&sid=1&slc_lang=en |
| Alternate Webpage(s) | http://bcn.iums.ac.ir/article-1-540-en.pdf |
| Alternate Webpage(s) | http://www.sid.ir/en/VEWSSID/J_pdf/1028620160309.pdf |
| PubMed reference number | 27563417v1 |
| Alternate Webpage(s) | https://doi.org/10.15412/J.BCN.03070309 |
| DOI | 10.15412/j.bcn.03070309 |
| Journal | Basic and clinical neuroscience |
| Volume Number | 7 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Analgesic brand of acetaminophen-phenyltoloxamine Analgesics Aspartic Acid Brain CFP gene CFP wt Allele Clarify Corpus striatum structure Cryoglobulin:PrThr:Pt:Ser:Ord:3D RT incubation Gene Expression Morphine N-Methyl-D-Aspartate Receptors N-Methylaspartate Neostriatum Opioids Prefrontal Cortex mg/kg |
| Content Type | Text |
| Resource Type | Article |
