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Macrophage colony-stimulating factor is indispensable for both proliferation and differentiation of osteoclast progenitors.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Tanaka, Sachiko Takahashi, Naoyuki Udagawa, Nobuyuki Tamura, Taka-Aki Akatsu, Takuhiko Stanley, E. Richard Kurokawa, Tomofumi Suda, Tatsuo |
| Copyright Year | 1993 |
| Abstract | The mechanism of action of macrophage colony-stimulating factor (M-CSF) in osteoclast development was examined in a co-culture system of mouse osteoblastic cells and spleen cells. In this co-culture, osteoclast-like multinucleated cells (MNCs) were formed within 6 d in response to 10 nM 1 alpha,25(OH)2D3 added only for the final 2 d of culture. Simultaneously adding hydroxyurea for the final 2 d completely inhibited proliferation of cultured cells without affecting 1 alpha,25(OH)2D3-stimulated MNC formation. Autoradiographic examination using [3H]-thymidine revealed that osteoclast progenitors primarily proliferated during the first 4 d, whereas their differentiation into MNCs occurred predominantly during the final 2 d of culture in response to 1 alpha,25(OH)2D3. When anti-M-CSF antibody or anti-M-CSF receptor antibody was added either for the first 4 d or for the final 2 d, the MNC formation was similarly inhibited. In co-cultures of normal spleen cells and osteoblastic cells obtained from op/op mice, which cannot produce functionally active M-CSF, the lack of M-CSF either for the first 4 d or for the final 2 d failed to form MNCs in response to 1 alpha,25(OH)2D3 added for the last 2 d. These results clearly indicate that M-CSF is indispensable for both proliferation of osteoclast progenitors and their differentiation into mature osteoclasts. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://dm5migu4zj3pb.cloudfront.net/manuscripts/116000/116179/JCI93116179.pdf |
| PubMed reference number | 8423223v1 |
| Volume Number | 91 |
| Issue Number | 1 |
| Journal | The Journal of clinical investigation |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Cell Differentiation process Coculture Techniques Colony-Stimulating Factors Fc Receptor Macrophage Colony-Stimulating Factor Osteoclasts Receptors, Colony-Stimulating Factor Spleen Tissue Stimulation (motivation) hydroxyurea osteoclast development |
| Content Type | Text |
| Resource Type | Article |
