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MMP profile in paired serum and synovial fluid samples of patients with rheumatoid arthritis.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Tchetverikov, Ilja Ronday, Herman K. El, Benno Van Verzijl, Nicole Tekoppele, J. M. Degroot, Jeroen Hanemaaijer, Roeland |
| Copyright Year | 2004 |
| Abstract | OBJECTIVE To analyse matrix metalloproteinases (MMPs) and tissue inhibitor-1 of MMPs (TIMP-1) levels in the systemic circulation and synovial fluid (SF) of patients with RA and to compare these levels with inflammatory and collagen degradation markers. METHODS ProMMP-1, -2, -3, -8, -9, TIMP-1, levels of MMP/alpha(2)-macroglobulin complexes, and collagen degradation products were measured by sandwich ELISA, activity assays, and HPLC in paired SF and serum samples from 15 patients with RA and 13 with OA. RESULTS MMPs were higher in SF of patients with RA than in OA or controls. MMP levels in SF of patients with OA were higher than in controls. In serum, levels of proMMP-3, -8 and -9 were higher in patients with RA than in OA or controls, whereas only proMMP-8 and -9 were higher in serum of patients with OA than in controls. A strong correlation was seen between serum and SF levels of MMP-8 and -9 in RA. Increased levels of MMP/alpha(2)-macroglobulin complexes indicated an MMP/TIMP imbalance in serum and SF in RA. SF hydroxyproline correlated significantly with SF levels of proMMP-9 in RA. CONCLUSIONS Systemic MMP-8 and -9 levels represent the situation in the inflamed joint; MMP-9 is likely to be involved in degradation of joint collagen. The hypothesis of MMP/TIMP imbalance in RA is strengthened. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ard.bmj.com/content/annrheumdis/63/7/881.full.pdf |
| PubMed reference number | 15194590v1 |
| Volume Number | 63 |
| Issue Number | 7 |
| Journal | Annals of the rheumatic diseases |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Enzyme-Linked Immunosorbent Assay Hydroxyproline Matrix Metalloproteinases Metalloproteases Patients Pro-Matrix Metalloproteinase-1 Rheumatoid Arthritis Synovial Fluid Tissue Inhibitor of Metalloproteinases collagen catabolic process |
| Content Type | Text |
| Resource Type | Article |
