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Identification of a membrane glycoprotein found primarily in the prelysosomal endosome compartment
| Content Provider | Semantic Scholar |
|---|---|
| Author | Park, Jie Eun Lopez, Jesus Maria Cluett, Edward B. |
| Copyright Year | 1991 |
| Abstract | Cells contain an intracellular compartment that serves as both the "prelysosomal" delivery site for newly synthesized lysosomal enzymes by the mannose 6-phosphate (Man6P) receptor and as a station along the endocytic pathway to lysosomes. We have obtained mAbs to a approximately 57-kD membrane glycoprotein, (called here plgp57), found predominantly in this prelysosomal endosome compartment. This conclusion is supported by the following results: (a) plgp57 was primarily found in a population of late endosomes that were located just distal to the 20 degrees C block site in the endocytic pathway to lysosomes (approximately 83% of the prelysosomes were positive for plgp57 but less than 5% of the early endosomes had detectable amounts of this marker); (b) plgp57 and the cation-independent (CI) Man6P receptor were located in many of the same intracellular vesicles; (c) plgp57 was found in the membranes of an acidic compartment; (d) immunoelectron microscopy showed that plgp57 was located in characteristic multilamellar- and multivesicular-type vacuoles believed to be prelysosomal endosomes; and (e) cell fractionation studies demonstrated that plgp57 was predominantly found in low density organelles which comigrated with late endosomes and CI Man6P receptors, and only approximately 10-15% of the antigen was found in high density fractions containing the majority of secondary lysosomes. These results indicate that plgp57 is a novel marker for a unique prelysosomal endosome compartment that is the site of confluence of the endocytic and biosynthetic pathways to lysosomes. |
| Starting Page | 245 |
| Ending Page | 255 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 1846371v1 |
| Volume Number | 112 |
| Journal | The Journal of cell biology |
| Alternate Webpage(s) | http://jcb.rupress.org/content/jcb/112/2/245.full.pdf |
| Alternate Webpage(s) | http://jcb.rupress.org/content/112/2/245.full.pdf |
| Alternate Webpage(s) | http://citeseerx.ist.psu.edu/viewdoc/download;jsessionid=4F6C92E97733B4609C193DC079A6CDB8?doi=10.1.1.319.930&rep=rep1&type=pdf |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Acids Anabolism Anatomical compartments Cations Endosomes Guanosine Diphosphate Mannose Leukemia, B-Cell Lysosomes Membrane Glycoproteins Tissue membrane Vacuole Vesicle (morphologic abnormality) |
| Content Type | Text |
| Resource Type | Article |
