GATA-1 testis activation region is essential for Sertoli cell-specific expression of GATA-1 gene in transgenic mouse.

Content Provider	Semantic Scholar
Author	Wakabayashi, Junko Yomogida, Kentaro Nakajima, Osamu Yoh, Keigyou Takahashi, Satoru Engel, James Douglas Ohneda, Kinuko Yamamoto, Masayuki
Copyright Year	2003
Abstract	BACKGROUND The erythroid transcription factor GATA-1 is also expressed in Sertoli cells of the testis. The testicular expression of GATA-1 is regulated in a developmental and spermatogenic stage-specific manner. To further clarify the regulatory mechanisms of testicular GATA-1 gene expression, we carried out transgenic reporter gene expression analyses. RESULTS We found that GATA-1 expression in Sertoli cells is markedly decreased concomitant with the emergence of elongated spermatids in the seminiferous tubules. Transgenic reporter mouse analyses revealed that a 15 kb GATA-1 genomic region is sufficient to recapitulate the gene expression profile in Sertoli cells. While the GATA-1 haematopoietic enhancer and the proximal first exon are included within the 15 kb genomic region, these regulatory elements are not essential for GATA-1 expression in Sertoli cells. Further analyses using deletion constructs revealed that a 1.5 kb region 5' to the GATA-1 haematopoietic enhancer is essential for gene expression in Sertoli cells and this region is referred to as the GATA-1 testis activation region. CONCLUSION These results thus demonstrated that the GATA-1 testis activation region is essential for Sertoli cell-specific expression of GATA-1 gene. The 15 kb genomic region is applicable and useful for the expression vector system specific for adult Sertoli cells in stage VII to IX.
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Alternate Webpage(s)	https://deepblue.lib.umich.edu/bitstream/handle/2027.42/71393/j.1365-2443.2003.00658.x.pdf;sequence=1
PubMed reference number	12839622v1
Volume Number	8
Issue Number	7
Journal	Genes to cells : devoted to molecular & cellular mechanisms
Language	English
Access Restriction	Open
Subject Keyword	Deletion Mutation Enhancer of transcription GATA1 gene GATA1 protein, human Gene Expression Hematopoietic System Leukemia, B-Cell Seminiferous tubule structure Spermatid TRANSCRIPTION FACTOR
Content Type	Text
Resource Type	Article