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Immobilization of GL-7-ACA acylase for the production of 7-ACA
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lee, Seung Kwon Park, Seung Won Chung, K. H. Kim, Seung Wook |
| Copyright Year | 2002 |
| Abstract | Glutaryl-7-aminocephalosporanic acid(GL-7-ACA) acylase is an important enzyme for the production of 7-ACA (7-aminocephalosporanic acid). For an efficient immobilization of GL-7-ACA acylase, various carriers were tested. A high-porous hydrophilic carrier (FPHA) among various carriers tested was found to be the best for the immobilization of GL-7-ACA acylase. In order to develop an effective immobilization method of GL-7-ACA acylase, the parameters that affect the immobilization of GL-7-ACA acylase were also investigated under different conditions of buffer solution and different concentrations of glutaraldehyde. The highest value of GL-7-ACA acylase activity (70 Unit/g-matrix) was obtained when immobilized with 1% glutaraldehyde in a 0.1 M Tris buffer (pH 8.0). Also, in order to enhance the activity of the immobilized GL-7-ACA acylase, unreacted aldehyde groups were quenched by reaction with a low molecular weight agent such as L-lysine after immobilization. The highest activity of immobilized GL-7-ACA acylase was obtained at 0.1% of L-lysine. The immobilized GL-7-ACA acylase was tested for long-term stability and it was found that the activity was retained at about 62% of the initial value after 72 times of reuse at 25 ‡C. |
| Starting Page | 261 |
| Ending Page | 266 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/BF02698411 |
| Alternate Webpage(s) | https://www.cheric.org/PDF/KJChE/KC19/KC19-2-0261.pdf |
| Alternate Webpage(s) | https://doi.org/10.1007/BF02698411 |
| Volume Number | 19 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
