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ELISA-based and Traditional Diagnosis Methods for Identification of Pseudomonas cichorii and Pseudomonas corrugata Causing Pith Necrosis on Tomato Plants
| Content Provider | Semantic Scholar |
|---|---|
| Author | Imriz, Gül Çinar, Özden |
| Copyright Year | 2016 |
| Abstract | Pseudomonas cichorii and Pseudomonas corrugata, the causal bacterial agents of tomato pith necrosis, may cause severe losses on tomato worldwide. In Turkey, P. cichorii and P. corrugata were observed on tomato in the Mediterranean Region. The typical disease symptoms of pathogens were characterized as wilting, dark blotches on tomato stems, browning and hollowing of pith in greenhouse tomato plants in Adana and Mersin provinces. In this study, traditional and ELISA (Enzyme-Linked ImmunoSorbent Assay)-based methods were performed for identification of isolates obtained from symptoms showing plants. In total, 15 of P. cichorii isolates and 1 of P. corrugata isolate were identified according to the diagnostic tests. In addition to traditional diagnosis methods, Indirect-ELISA that was made with pure bacterial cultures was evaluated as capable and reliable diagnosis method of rapid resulting for both pathogenic bacteria. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://dergipark.gov.tr/download/article-file/253686 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |