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Eicosanoid Diversity of Stony Corals
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lõhelaid, Helike Samel, Nigulas |
| Copyright Year | 2018 |
| Abstract | Oxylipins are well-established lipid mediators in plants and animals. In mammals, arachidonic acid (AA)-derived eicosanoids control inflammation, fever, blood coagulation, pain perception and labor, and, accordingly, are used as drugs, while lipoxygenases (LOX), as well as cyclooxygenases (COX) serve as therapeutic targets for drug development. In soft corals, eicosanoids are synthesized on demand from AA by LOX, COX, and catalase-related allene oxide synthase-lipoxygenase (cAOS-LOX) and hydroperoxide lyase-lipoxygenase (cHPL-LOX) fusion proteins. Reef-building stony corals are used as model organisms for the stress-related genomic studies of corals. Yet, the eicosanoid synthesis capability and AA-derived lipid mediator profiles of stony corals have not been determined. In the current study, the genomic and transcriptomic data about stony coral LOXs, AOS-LOXs, and COXs were analyzed and the eicosanoid profiles and AA metabolites of three stony corals, Acropora millepora, A. cervicornis, and Galaxea fascicularis, were determined by reverse-phase high-performance liquid chromatography (RP-HPLC) coupled with MS-MS and a radiometric detector. Our results confirm that the active LOX and AOS-LOX pathways are present in Acropora sp., which correspond to the genomic/sequence data reported earlier. In addition, LOX, AOS-LOX, and COX products were detected in the closely related species G. fascicularis. In conclusion, the functional 8R-LOX and/or AOS-LOX pathways are abundant among corals, while COXs are restricted to certain soft and stony coral lineages. |
| Starting Page | 681 |
| Ending Page | 697 |
| Page Count | 17 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 29301345v1 |
| Alternate Webpage(s) | https://doi.org/10.3390/md16010010 |
| DOI | 10.3390/md16010010 |
| Journal | Marine drugs |
| Volume Number | 16 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adams Oliver syndrome Arachidonic Acid Blood Coagulation Disorders CATALASE Coral Detectors Eicosanoids Fever Glycyrrhiza uralensis Hydrogen Peroxide Inflammation Lipoxygenase Lipoxygenases Liquid Chromatography Mammals Mediator brand of benfluorex hydrochloride Oxylipins Pain Perception Prostaglandin-Endoperoxide Synthase drug development hydroperoxide lyase |
| Content Type | Text |
| Resource Type | Article |
