NDLI: Rapid detection of methicillin resistance in Staphylococcus aureus isolates by the MRSA-screen latex agglutination test.

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Rapid detection of methicillin resistance in Staphylococcus aureus isolates by the MRSA-screen latex agglutination test.

Content Provider	Semantic Scholar
Author	Leeuwen, Willem B. Van Pelt, Cindy Van Luijendijk, Arjen Verbrugh, Henri A. Goessens, Wil H. F.
Copyright Year	1999
Abstract	The slide agglutination test MRSA-Screen (Denka Seiken Co., Niigata, Japan) was compared with the mecA PCR ("gold standard") for the detection of methicillin resistance in Staphylococcus aureus. The MRSA-Screen test detected the penicillin-binding protein 2a (PBP2a) antigen in 87 of 90 genetically diverse methicillin-resistant S. aureus (MRSA) stock culture strains, leading to a sensitivity of 97%. The three discrepant MRSA strains displayed positive results only after induction of the mecA gene by exposure to methicillin. Both mecA PCR and MRSA-Screen displayed negative results among the methicillin-susceptible S. aureus strains (n = 106), as well as for Micrococcus spp. (n = 10), members of the family Enterobacteriaceae (n = 10), Streptococcus pneumoniae (n = 10), and Enterococcus spp. (n = 10) (specificity = 100%). Producing the same PBP2a antigen, all 10 methicillin-resistant Staphylococcus epidermidis strains score positived in both the latex test and the mecA PCR. Consequently, the MRSA-Screen test should be applied only after identification of the MRSA strain to the species level to rule out coagulase-negative staphylococci. In conclusion, due to excellent specificity and sensitivity the MRSA-Screen latex test has the potential to be successfully used for routine applications in the microbiology laboratory.
File Format	PDF HTM / HTML
DOI	10.1128/jcm.37.9.3029-3030.1999
PubMed reference number	10449498
Journal	Medline
Volume Number	37
Issue Number	9
Alternate Webpage(s)	https://repub.eur.nl/pub/9150/10449498.pdf
Alternate Webpage(s)	http://jcm.asm.org/content/37/9/3029.full.pdf
Alternate Webpage(s)	https://doi.org/10.1128/jcm.37.9.3029-3030.1999
Journal	Journal of clinical microbiology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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