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Routine and timely sub-picoNewton force stability and precision for biological applications of atomic force microscopy.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Churnside, Allison B. Sullan, Ruby May Arana Nguyen, Duc Minh Case, S. O. Bull, Matthew S. King, Gavin Morgan Perkins, Thomas T. |
| Copyright Year | 2012 |
| Abstract | Force drift is a significant, yet unresolved, problem in atomic force microscopy (AFM). We show that the primary source of force drift for a popular class of cantilevers is their gold coating, even though they are coated on both sides to minimize drift. Drift of the zero-force position of the cantilever was reduced from 900 nm for gold-coated cantilevers to 70 nm (N = 10; rms) for uncoated cantilevers over the first 2 h after wetting the tip; a majority of these uncoated cantilevers (60%) showed significantly less drift (12 nm, rms). Removing the gold also led to ∼10-fold reduction in reflected light, yet short-term (0.1-10 s) force precision improved. Moreover, improved force precision did not require extended settling; most of the cantilevers tested (9 out of 15) achieved sub-pN force precision (0.54 ± 0.02 pN) over a broad bandwidth (0.01-10 Hz) just 30 min after loading. Finally, this precision was maintained while stretching DNA. Hence, removing gold enables both routine and timely access to sub-pN force precision in liquid over extended periods (100 s). We expect that many current and future applications of AFM can immediately benefit from these improvements in force stability and precision. |
| File Format | PDF HTM / HTML |
| DOI | 10.1021/nl301166w |
| PubMed reference number | 22694769 |
| Journal | Medline |
| Volume Number | 12 |
| Issue Number | 7 |
| Alternate Webpage(s) | https://core.ac.uk/download/pdf/82825377.pdf |
| Alternate Webpage(s) | https://jila.colorado.edu/perkins/sites/default/files/pdf/Churnside%20et%20al%202012%20Routine%20and%20Timely%20sub-picoNewtwon%20force%20stability%20and%20precision%20for%20bioAFM.pdf |
| Alternate Webpage(s) | https://doi.org/10.1021/nl301166w |
| Journal | Nano letters |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
