Adaptation of energy metabolism in breast cancer brain metastases.

Content Provider	Semantic Scholar
Author	Chen, Emily I. Hewel, Johannes Krueger, Joseph S. Tiraby, Claire Weber, Martin R. Kralli, Anastasia Becker, Katja Yates, John R. Felding-Habermann, Brunhilde
Copyright Year	2007
Abstract	Brain metastases are among the most feared complications in breast cancer, as no therapy exists that prevents or eliminates breast cancer spreading to the brain. New therapeutic strategies depend on specific knowledge of tumor cell properties that allow breast cancer cell growth within the brain tissue. To provide information in this direction, we established a human breast cancer cell model for brain metastasis based on circulating tumor cells from a breast cancer patient and variants of these cells derived from bone or brain lesions in immunodeficient mice. The brain-derived cells showed an increased potential for brain metastasis in vivo and exhibited a unique protein expression profile identified by large-scale proteomic analysis. This protein profile is consistent with either a selection of predisposed cells or bioenergetic adaptation of the tumor cells to the unique energy metabolism of the brain. Increased expression of enzymes involved in glycolysis, tricarboxylic acid cycle, and oxidative phosphorylation pathways suggests that the brain metastatic cells derive energy from glucose oxidation. The cells further showed enhanced activation of the pentose phosphate pathway and the glutathione system, which can minimize production of reactive oxygen species resulting from an enhanced oxidative metabolism. These changes promoted resistance of brain metastatic cells to drugs that affect the cellular redox balance. Importantly, the metabolic alterations are associated with strongly enhanced tumor cell survival and proliferation in the brain microenvironment. Thus, our data support the hypothesis that predisposition or adaptation of the tumor cell energy metabolism is a key element in breast cancer brain metastasis, and raise the possibility of targeting the functional differentiation in breast cancer brain lesions as a novel therapeutic strategy.
Starting Page	17
Ending Page	24
Page Count	8
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://cancerres.aacrjournals.org/content/canres/67/4/1472.full.pdf
PubMed reference number	17308085v1
Volume Number	67
Issue Number	4
Journal	Cancer research
Language	English
Access Restriction	Open
Subject Keyword	Acclimatization Bioenergetics Bone Tissue Breast Cancer Cell Cancer Cell Growth Cancer Patient Cell Respiration Cell Survival Citric Acid Cycle Energy Metabolism Glucose Glutathione Glycolysis Immunologic Deficiency Syndromes Mammary Neoplasms Metabolic Process, Cellular Metastatic malignant neoplasm to brain Neoplastic Cell Oxidative Phosphorylation Pentose Phosphate Pathway Pentosephosphates Pentoses Reactive Oxygen Species Tricarboxylic Acids cellular targeting oxidation pediatric intracranial germ cell brain tumor protein expression
Content Type	Text
Resource Type	Article