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Bacterial Polysaccharides Suppress Induced Innate Immunity by Calcium Chelation
| Content Provider | Semantic Scholar |
|---|---|
| Author | Aslam, Shazia N. Newman, Mari-Anne Erbs, Gitte Morrissey, Kate L. Chinchilla, Delphine Boller, Thomas Jensen, Tina Tandrup Castro, Cristina De Ieranò, Teresa Molinaro, Antonio Jackson, Robert W. Knight, Marc R. Cooper, Richard M. |
| Copyright Year | 2008 |
| Abstract | Bacterial pathogens and symbionts must suppress or negate host innate immunity. However, pathogens release conserved oligomeric and polymeric molecules or MAMPs (Microbial Associated Molecular Patterns), which elicit host defenses [1], [2] and [3]. Extracellular polysaccharides (EPSs) are key virulence factors in plant and animal pathogenesis, but their precise function in establishing basic compatibility remains unclear [4], [5], [6] and [7]. Here, we show that EPSs suppress MAMP-induced signaling in plants through their polyanionic nature [4] and consequent ability to chelate divalent calcium ions [8]. In plants, Ca2+ ion influx to the cytosol from the apoplast (where bacteria multiply [4], [5] and [9]) is a prerequisite for activation of myriad defenses by MAMPs [10]. We show that EPSs from diverse plant and animal pathogens and symbionts bind calcium. EPS-defective mutants or pure MAMPs, such as the flagellin peptide flg22, elicit calcium influx, expression of host defense genes, and downstream resistance. Furthermore, EPSs, produced by wild-type strains or purified, suppress induced responses but do not block flg22-receptor binding in Arabidopsis cells. EPS production was confirmed in planta, and the amounts in bacterial biofilms greatly exceed those required for binding of apoplastic calcium. These data reveal a novel, fundamental role for bacterial EPS in disease establishment, encouraging novel control strategies. |
| Starting Page | 1078 |
| Ending Page | 1083 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/j.cub.2008.06.061 |
| PubMed reference number | 18639458 |
| Journal | Medline |
| Volume Number | 18 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/S096098220800818X |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/S096098220800818X |
| Alternate Webpage(s) | http://download.cell.com/current-biology/mmcs/journals/0960-9822/piis096098220800818x.mmc1.pdf |
| Alternate Webpage(s) | https://doi.org/10.1016/j.cub.2008.06.061 |
| Journal | Current Biology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
