NDLI: Culture of the marine diatom Phaeodactylum tricornutum with different nitrogen sources: Growth, nutrient conversion and biochemical composition

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Culture of the marine diatom Phaeodactylum tricornutum with different nitrogen sources: Growth, nutrient conversion and biochemical composition

Content Provider	Semantic Scholar
Author	Fidalgo, J. A. Pérez Cid, Ángeles Abalde, Julio Herrero, Concepción
Copyright Year	1995
Abstract	Cultures of the marine diatom Phaeodaetylum trieornutum were grown in nitrate, nitrite, ammonia or urea media. Nitrogen transformation efficiencies were close to 100% in cultures with nitrate, nitrite or urea, with productivities in dry biomass between 2.2 g.l-1 (urea) and 2.8 g.t 1 (nitrate). However growth in ammonia cultures ceased due to a drop in pH of the medium. The biochemical profile was more affected by the growth phase than by the N source. Cellular contents of chlorophylls a and e decreased and the chlorophyll ale ratio increased when cultures entered the stationary phase. Protein and RNA were higher during exponential growth (about 30% and 8% ash free dry weight (AFDW), respectively), but carbo hydrates increased up to 220% in the stationary phase. Although significant differences were observed in cellular contents, the biochemical composition (as % AFDW) of the nitrate, nitrite and urea-grown cells was very similar. The highest total fatty acids and polyunsaturated fatty acids (PUFAs) contents in the stationary phase were obtained in the urea-grown cells, with an eicosapentaenoic acid (EPA) content of 26.8 mg.g- 1 of dry biomass.
Starting Page	165
Ending Page	173
Page Count	9
File Format	PDF HTM / HTML
Volume Number	36
Alternate Webpage(s)	http://www.vliz.be/imisdocs/publications/287515.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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