An inactive pool of GSK-3 at the leading edge of growth cones is implicated in Semaphorin 3A signaling

Content Provider	Semantic Scholar
Author	Eickholt, Britta Johanna Doherty, Patrick
Copyright Year	2002
Abstract	Glycogen synthase kinase (GSK)-3 is a serine/threonine kinase that has been implicated in several aspects in embryonic development and several growth factor signaling cascades. We now report that an inactive phosphorylated pool of the enzyme colocalizes with F-actin in both neuronal and nonneuronal cells. Semaphorin 3A (Sema 3A), a molecule that inhibits axonal growth, activates GSK-3 at the leading edge of neuronal growth cones and in Sema 3A-responsive human breast cancer cells, suggesting that GSK-3 activity might play a role in coupling Sema 3A signaling to changes in cell motility. We show that three different GSK-3 antagonists (LiCl, SB-216763, and SB-415286) can inhibit the growth cone collapse response induced by Sema 3A. These studies reveal a novel compartmentalization of inactive GSK-3 in cells and demonstrate for the first time a requirement for GSK-3 activity in the Sema 3A signal transduction pathway.
Starting Page	211
Ending Page	217
Page Count	7
File Format	PDF HTM / HTML
PubMed reference number	11956225v1
Volume Number	157
Journal	The Journal of cell biology
Alternate Webpage(s)	http://jcb.rupress.org/content/jcb/157/2/211.full.pdf
Alternate Webpage(s)	http://jcb.rupress.org/content/157/2/211.full.pdf
Language	English
Access Restriction	Open
Subject Keyword	Breast Cancer Cell Embryonic Development F-Actin Glycogen Synthase Kinase 3 Glycogen Synthase Kinases Growth Cones Growth Factor Inactive - Biochemical Activity Level Lithium Chloride MARK2 gene Mammary Neoplasms Maxillary right first molar abutment Neurogenesis Physical Inactivity Protein-Serine-Threonine Kinases Retinal Cone SB 216763 SB 415286 Semaphorin-3A Semaphorins Signal Transduction Pathways Threonine cell motility eIF-2 Kinase
Content Type	Text
Resource Type	Article