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A Micropropagation System for Cloning of Hemp (cannabis Sativa L.) by Shoot Tip Culture
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wang, Ren He, Lisi Xia, Bing Tong, Jin-Feng Li, Ning Peng, Feng |
| Copyright Year | 2009 |
| Abstract | This study describes the standardization of an efficient in vitro propagation and hardening procedure for obtaining plantlets from shoot tips of Hemp (Cannabis sativa L.). Hemp seedlings were germinated on half-strength 1/2 MS medium supplemented with 10 g·L -1 sucrose, 5.5 g·L -1 agar at a pH of 6.8 under light for 16 h per day. MS medium containing 0.2 mg·L -1 TDZ, 0.1 mg·L -1 NAA supported the maximal auxiliary bud multiplication rate of 3.22 per shoot tip. The proliferated buds were successfully rooted on MS medium supplemented with 0.1 mg·L -1 IBA and 0.05 mg·L -1 NAA resulting in 85% of the plantlets rooting. The procedure requires a 54 days cycle for the In vitro clonal propagation (14 days for shoot multiplication and 40 days for root induction) which includes 35-42 days for acclimatized plantlet production. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.pakbs.org/pjbot/PDFs/41(2)/PJB41(2)603.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
