NDLI: Efeito do congelamento na contagem de Salmonella enteritidis pelo método do número mais provável (NMP) em cecos de frangos de corte

Please wait, while we are loading the content...

Efeito do congelamento na contagem de Salmonella enteritidis pelo método do número mais provável (NMP) em cecos de frangos de corte

Content Provider	Semantic Scholar
Author	Vieira, Vanessa Rodrigues Nascimento, Vladimir Pinheiro Do Borsoi, Anderlise Santos, Luciana Ruschel Dos
Copyright Year	2007
Abstract	O objetivo do presente trabalho foi avaliar o comportamento quantitativo sob o estresse de diferentes periodos de congelamento a temperatura de -20oC de Salmonella Enteritidis inoculada em cecos de frangos de corte, atraves do metodo do Numero Mais Provavel (NMP). Foram coletados 60 cecos na linha de producao de uma industria avicola e realizada a contagem de salmonelas pelo NMP. Foram realizados 4 tratamentos, sendo T1: material nao congelado e T2, T3 e T4 amostras congeladas por 1, 3 e 7 dias a –20oC, respectivamente. No primeiro tratamento foram obtidos os numeros maximos para presenca de celulas de Salmonella por NMP (>110 cel/g) em todas as amostras. Nos cecos congelados por 1 e 3 dias, sete amostras apresentaram diminuicao do NMP e, no congelamento por 7 dias, somente cinco amostras diminuiram a contagem pelo NMP. Nao houve diferenca significativa entre a reducao bacteriana e os tempos de congelamentos das amostras nos 4 tratamentos, entretanto, amostras nao congeladas obtiveram maiores contagens pelo NMP frente aquelas congeladas. O congelamento de amostras pode interferir na recuperacao e contagem de celulas de salmonelas, influenciando na identificacao da real contaminacao existente por ocasiao do periodo de coleta das mesmas. Palavras chave: Salmonella enteritidis, contagem por NMP, frangos de corte, cecos ABSTRACT The aim of this study was to evaluate the Salmonella enteritidis quantitative behavior, through the Most Probable Number (MPN) method, under different stress time conditions, at –20oC. Samples of 60 ceca from broiler were collected, obtained in the production line from a poultry industry. Ceca were divided in 4 treatments, as follows: T1: samples not frozen and T2, T3 e T4 samples frozen for 1, 3 and 7 days at –20oC, respectively. The results showed in the first treatment the maximum numbers of the presence of Salmonella cells for NMP (>110 cel/g) in all samples. In the ceca frozen for 1 and 3 days, seven samples showed a decrease in NMP counting. The samples frozen by 7 days, only five samples had a counting decrease by NMP. Samples frozen and non-frozen didn’t show significant difference reduction in MPN among freezing times. However, the non-frozen samples obtained higher counting by MPN than ones submitted to -20o C procedure. The samples submitted to frozen procedures can present some difficulties to recover and count microbial cells, so this way would bring damages to reflect the real situation at time of acquiring the samples. Key words: Salmonella enteritidis, quantification by MPN, broiler, ceca.
File Format	PDF HTM / HTML
Volume Number	14
Alternate Webpage(s)	http://revistaseletronicas.pucrs.br/ojs/index.php/fzva/article/download/2505/1964
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in