NDLI: Structural determinants of synaptobrevin 2 function in synaptic vesicle fusion.

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Structural determinants of synaptobrevin 2 function in synaptic vesicle fusion.

Content Provider	Semantic Scholar
Author	Deák, Ferenc Shin, Ok-Ho Kavalali, Ege T. Südhof, Thomas C.
Copyright Year	2006
Abstract	Deletion of synaptobrevin/vesicle-associated membrane protein, the major synaptic vesicle soluble N-ethylmaleimide-sensitive factor attachment protein receptor (R-SNARE), severely decreases but does not abolish spontaneous and evoked synaptic vesicle exocytosis. We now show that the closely related R-SNARE protein cellubrevin rescues synaptic transmission in synaptobrevin-deficient neurons but that deletion of both cellubrevin and synaptobrevin does not cause a more severe decrease in exocytosis than deletion of synaptobrevin alone. We then examined the structural requirements for synaptobrevin to function in exocytosis. We found that substituting glutamine for arginine in the zero-layer of the SNARE motif did not significantly impair synaptobrevin-dependent exocytosis, whereas insertion of 12 or 24 residues between the SNARE motif and transmembrane region abolished the ability of synaptobrevin to mediate Ca2+-evoked exocytosis. Surprisingly, however, synaptobrevin with the 12-residue but not the 24-residue insertion restored spontaneous release in synaptobrevin-deficient neurons. Our data suggest that synaptobrevin mediates Ca2+-triggered exocytosis by tight coupling of the SNARE motif to the transmembrane region and hence forcing the membranes into close proximity for fusion. Furthermore, the fusion reactions underlying evoked and spontaneous release differ mechanistically.
File Format	PDF HTM / HTML
DOI	10.1523/JNEUROSCI.5272-05.2006
PubMed reference number	16793874
Journal	Medline
Volume Number	26
Issue Number	25
Alternate Webpage(s)	http://www.jneurosci.org/content/jneuro/26/25/6668.full.pdf
Alternate Webpage(s)	https://doi.org/10.1523/JNEUROSCI.5272-05.2006
Journal	The Journal of neuroscience : the official journal of the Society for Neuroscience
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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