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Influence of hyperglycemia on oxidative stress and matrix metalloproteinase-9 activation after focal cerebral ischemia/reperfusion in rats: relation to blood-brain barrier dysfunction.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kamada, Hiroshi Yu, Fengshan Nito, Chikako Chan, Pak Hoo |
| Copyright Year | 2007 |
| Abstract | BACKGROUND AND PURPOSE Hyperglycemia is linked to a worse outcome after ischemic stroke. Among the manifestations of brain damage caused by ischemia are blood-brain barrier (BBB) disruption and edema formation. Oxidative stress and matrix metalloproteinase-9 (MMP-9) activation are implicated in BBB dysfunction after ischemia/reperfusion injury. Our present study was designed to clarify the relation among hyperglycemia, oxidative stress, and MMP-9 activation associated with BBB dysfunction after transient focal cerebral ischemia (tFCI). METHODS We used a model of 60 minutes of middle cerebral artery occlusion on the following animals: normoglycemic wild-type rats, wild-type rats with hyperglycemia induced by streptozotocin, and human copper/zinc superoxide dismutase (SOD1) transgenic rats with streptozotocin-induced hyperglycemia. We evaluated edema volume, Evans blue leakage, and oxidative stress, such as the carbonyl groups and oxidized hydroethidine (HEt), SOD activity, and gelatinolytic activity, including MMP-9. RESULTS Hyperglycemia significantly increased edema volume and Evans blue leakage. Moreover, it enhanced the levels of the carbonyl groups, the oxidized HEt signals, and MMP-9 activity after tFCI without alteration in SOD activity. Gelatinolytic activity and oxidized HEt signals had a clear spatial relation in the hyperglycemic rats. SOD1 overexpression reduced the hyperglycemia-enhanced Evans blue leakage and MMP-9 activation after tFCI. CONCLUSIONS Hyperglycemia increases oxidative stress and MMP-9 activity, exacerbating BBB dysfunction after ischemia/reperfusion injury. Superoxide overproduction may be a causal link among hyperglycemia, MMP-9 activation, and BBB dysfunction. |
| File Format | PDF HTM / HTML |
| DOI | 10.1161/01.STR.0000258041.75739.cb |
| PubMed reference number | 17272778 |
| Journal | Medline |
| Volume Number | 38 |
| Issue Number | 3 |
| Alternate Webpage(s) | http://stroke.ahajournals.org/content/strokeaha/38/3/1044.full.pdf?download=true |
| Alternate Webpage(s) | https://doi.org/10.1161/01.STR.0000258041.75739.cb |
| Journal | Stroke |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
