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Characterization of cytoplasmic and nuclear genomes in the colorless alga Polytoma. II. General characterization of organelle nucleic acids
| Content Provider | Semantic Scholar |
|---|---|
| Author | Siu, Chi-Hung Chiang, Kwen-Sheng Swift, Hewson |
| Copyright Year | 2004 |
| Abstract | DNA isolated from purified nuclei of Polytoma obtusum has a buoyant density of 1.711 g/ml in CsCl, a Tm of 91.3° C in SSC, and a G + C content of 52.5% as determined by base composition analysis. Thermal dissociation and reassociation studies indicated that this nuclear DNA contains a considerable amount of heterogeneity. Under appropriate reannealing conditions for denatured DNA, about 15% of the DNA reannealed to form a satellite peak at a density of 1.711 g/ml within one hour. Native DNA fractions of different average buoyant densities, ranging from 1.723 to 1.708 g/ml were also obtained in a preparative CsCl gradient, indicating the presence of intermolecular heterogeneity at a molecular size of 8.5×106 daltons. The nuclear DNA reassociated as three distinct classes. The very fast species constituted about 20 % of the total hyperchromicity, the class of intermediate rate comprised roughly 10% of the nuclear DNA, while the remaining 70% consisted of unique sequences. The haploid genome set was estimated by renaturation kinetics studies to contain 5.0×1010 daltons of DNA or 7.5×107 nucleotide pairs. The analytical complexity of the total nuclear genome was found to be 9.35×1010 daltons, thus indicating that vegetative cells of P. obtusum are diploid. |
| Starting Page | 371 |
| Ending Page | 382 |
| Page Count | 12 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/BF00284864 |
| PubMed reference number | 1262395 |
| Journal | Medline |
| Volume Number | 69 |
| Journal | Chromosoma |
| Alternate Webpage(s) | http://citeseerx.ist.psu.edu/viewdoc/download;jsessionid=A1607686D6311FFBC03A7D9966BFADB1?doi=10.1.1.281.6632&rep=rep1&type=pdf |
| Alternate Webpage(s) | http://ftp.ncbi.nlm.nih.gov/pub/pmc/30/12/jc692352.PMC2109689.pdf |
| Alternate Webpage(s) | http://jcb.rupress.org/content/jcb/69/2/371.full.pdf |
| Alternate Webpage(s) | https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f9/81/jc692371.PMC2109693.pdf |
| Alternate Webpage(s) | https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6a/3a/jc692383.PMC2109691.pdf |
| Alternate Webpage(s) | https://doi.org/10.1007/BF00284864 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
