Regulatory volume increase (RVI) and apoptotic volume decrease (AVD) in U937 cells in hypertonic medium

Content Provider	Semantic Scholar
Author	Yurinskaya, Valentina Rubashkin, Andrey A. Shirokova, Anna Vereninov, Alexey
Copyright Year	2011
Abstract	Changes in intracellular water, K+ and Na+ of U937 cells incubated in hyperosmolar medium supplemented with 200 mM sucrose have been studied. Cells were stained with acrydine orange, ethydium bromide, APOPercentage dye, which marks the phosphatidyl serine distribution on the plasma membrane; and FLICA polycaspase fluorescent dye. It was found that cell shrinkage produced by direct osmotic effect induced both a regulatory volume increase and apoptotic volume decrease. The regulatory volume increase dominated at the early stage, whereas apoptotic volume decrease prevailed at the later stage. Therefore, U937 cells were capable of triggering apoptosis and apoptotic volume decrease, despite the unimpaired regulatory volume increase response, and the current opinion that the dysfunction of the regulatory volume increase is a prerequisite for apoptosis and apoptotic volume decrease (Subramanyam et al., 2010) should be revised. It is concluded that the apoptotic volume decrease plays a significant role in preventing osmotic lysis in apoptotic cells, rather than in initiating apoptosis.
Starting Page	487
Ending Page	494
Page Count	8
File Format	PDF HTM / HTML
DOI	10.1134/S1990519X11050129
Volume Number	5
Alternate Webpage(s)	http://www.cytspb.rssi.ru/lab_vereninov/Yurinskaya%20RVI-AVD%20CellTissue%20Biol%202011.pdf
Alternate Webpage(s)	https://doi.org/10.1134/S1990519X11050129
Journal	Cell and Tissue Biology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article