NDLI: Nested Polymerase Chain Reaction and Prenatal Detection of Mycobacterium avium Subspecies paratuberculosis ( Map ) in Bovine Allantoic Fluid and Fetuses

Content Provider	Semantic Scholar
Author	Buergelt, Claus D. Williams, Elliot Monif, Gilles R. G. Pinedo, Pablo Decker, Joshua H.
Copyright Year	2006
Abstract	Eleven pregnant Holstein cows were subjected to nested polymerase chain reaction (nPCR) testing for evidence of in utero transmission with Mycobacterium avium subspecies paratuberculosis. Six dams (55%) were shown by nPCR to have positive PCR products in blood and/or milk. Tissues or fluid from fetuses were positive on 36% of the pregnancies; 2 of 4 placentomes tested gave positive PCR reaction products and 1 of 11 (9%) allantoic fluids. All pregnant cows were proven paratuberculosis positive through necropsy and microscopic examination. A percutanous technique on the standing pregnant cow is described for antemortem collection of allantoic fluid for PCR testing.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.jarvm.com/articles/Vol4Iss3/Buergelt.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in