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Autoreactive T cells to platelet GPIIb-IIIa in immune thrombocytopenic purpura. Role in production of anti-platelet autoantibody.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kuwana, Masataka Kaburaki, Junichi Ikeda, Yoshiou |
| Copyright Year | 1998 |
| Abstract | T cell proliferative responses to platelet membrane GPIIb-IIIa were examined in 14 patients with chronic immune thrombocytopenic purpura (ITP), 7 systemic lupus erythematosus (SLE) patients with or without thrombocytopenia, and 10 healthy donors. Although peripheral blood T cells from all subjects failed to respond to the protein complex in its native state, reduced GPIIb-IIIa stimulated T cells from three ITP patients and one SLE patient with thrombocytopenia, and tryptic peptides of GPIIb-IIIa stimulated T cells from nearly all subjects. The specificity of the responses for GPIIb-IIIa was confirmed by activation of GPIIb-IIIa-primed T cells by a recombinant GPIIbalpha fragment in secondary cultures. Characterization of T cell response induced by modified GPIIb-IIIa showed that the response was restricted by HLA-DR, the responding T cells had a CD4(+) phenotype, and the proliferation was accelerated only in ITP patients, suggesting in vivo activation of these T cells. In vitro IgG anti-GPIIb-IIIa synthesis in PBMC cultures was induced by modified GPIIb-IIIa specifically in ITP patients with platelet-associated anti-GPIIb-IIIa antibody. Anti-GPIIb-IIIa antibody produced in supernatants was absorbed by incubation with normal platelets. In summary, CD4(+) and HLA-DR-restricted T cells to GPIIb-IIIa are involved in production of anti-platelet autoantibody in ITP patients and are related to the pathogenic process in chronic ITP. |
| File Format | PDF HTM / HTML |
| DOI | 10.1172/JCI4238 |
| Alternate Webpage(s) | https://dm5migu4zj3pb.cloudfront.net/manuscripts/4000/4238/cache/4238.1-20140626150556-covered-253bed37ca4c1ab43d105aefdf7b5536.pdf |
| Alternate Webpage(s) | http://dm5migu4zj3pb.cloudfront.net/manuscripts/4000/4238/JCI9804238.pdf |
| PubMed reference number | 9769332 |
| Alternate Webpage(s) | https://doi.org/10.1172/JCI4238 |
| Journal | Medline |
| Volume Number | 102 |
| Issue Number | 7 |
| Journal | The Journal of clinical investigation |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
