Loading...
Please wait, while we are loading the content...
Similar Documents
Astilbin Inhibits the Activity of Sortase A from Streptococcus mutans
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wang, Junxian Jing, Shisong Dong, Haisi Wang, Dacheng Wang, Tiedong |
| Copyright Year | 2019 |
| Abstract | Streptococcus mutans (S. mutans) is the primary etiological agent of dental caries. The S. mutans enzyme sortase A (SrtA) is responsible for anchoring bacterial cell wall surface proteins involved in host cell attachment and biofilm formation. Thus, SrtA is an attractive target for inhibiting dental caries caused by S. mutans-associated acid fermentation. In this study, we observed that astilbin, a flavanone compound extracted from Rhizoma Smilacis Glabrae, has potent inhibitory activity against the S. mutans SrtA, with an IC50 of 7.5 μg/mL. In addition, astilbin was proven to reduce the formation of biofilm while without affecting the growth of S. mutans. The results of a molecular dynamics simulation and a mutation analysis revealed that the Arg213, Leu111, and Leu116 of SrtA are important for the interaction between SrtA and astilbin. The results of this study demonstrate the potential of using astilbin as a nonbactericidal agent to modulate pathogenicity of S. mutans by inhibiting the activity of SrtA. |
| File Format | PDF HTM / HTML |
| DOI | 10.3390/molecules24030465 |
| Alternate Webpage(s) | https://res.mdpi.com/molecules/molecules-24-00465/article_deploy/molecules-24-00465.pdf?attachment=1&filename= |
| PubMed reference number | 30696091 |
| Alternate Webpage(s) | https://doi.org/10.3390/molecules24030465 |
| Journal | Medline |
| Volume Number | 24 |
| Journal | Molecules |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
