Loading...
Please wait, while we are loading the content...
Similar Documents
Characterisation of Acidic and Basic Apoplastic Peroxidases from Needles of Norway Spruce (Picea abies, L., Karsten) with Respect to Lignifying Substrates
| Content Provider | Semantic Scholar |
|---|---|
| Author | Otter, Tilman Polle, Andrea |
| Copyright Year | 1997 |
| Abstract | Acidic and basic peroxidases, termed as POD-A and POD-B, were isolated from the apoplastic space of spruce (Picea abies, L.) needles and purified by acetone precipitation and anion exchange chromatography to apparent homogeneity. The molecular masses of POD-A and POD-B were 39.6 and 29.0 kDa, respectively. The pH optimum of both isozymes ranged from 4.5 to 6. The apparent Km values of POD-A and POD-B were 460 and 210 /M for coniferyl alcohol. Both isozymes acted also as NADH oxidases with apparent ^-values of 103 fM (POD-A) and 70 fM (POD-B). NAD + but not NADH was found in the apoplastic space of lignifying needles. Based on the lignification rate, the contents and kinetic properties of PODs, NADH oxidation by POD is not the major source of H2O2 required for lignin polymerisation. |
| Starting Page | 595 |
| Ending Page | 602 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| DOI | 10.1093/oxfordjournals.pcp.a029209 |
| Volume Number | 38 |
| Alternate Webpage(s) | https://watermark.silverchair.com/38-5-595.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAk4wggJKBgkqhkiG9w0BBwagggI7MIICNwIBADCCAjAGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMDrjOmThsBEjakgOqAgEQgIICAdQOMl7b_Aq8CeR0FTtnYAeMlNIy2txD7hXuLInE_QXCmHknjSDGr4HoCz05sAGNuHY5IVye6q5yapEJXwWZflhCLYu0162NrBYTxvTYvRG20qaVKmy0IhzkBPS2F0_KOriR3OSUWQWoVBCaOhkTVdYizJf2Z-wtC3zvWYV9FYA9zih1CZ22UYsFeJiyO5bTRnhzdQEPukd_I7GbanYsQDDSlJMHRrr_litvuaU75Id3N2lLSEDFy81j1i1WZqBoOPWbF8EySwblPwe8sFBXFABw7mfKWSl4Gnn0xfwmrJvuZjCbuCr0CtEkuTVALJ1YaSzVAtVp7HzQUbdmnNy_eAe5w5Jvij1781CrXEmRJ0LBmD3dHuKbejvDGoz1x3vpvMNeN4q1dyQCOz-cvVpELVmyWyMls81bcT7WGtHm5-maUMBbezBATAYbGdWVr5LtZs4SJt_CrE1x2_z0RNzH6vH2_8hXtu1qP9F5Aq4-EUd9ZAkaiI_1lmWmJiWxKwynEzer-EiuoMd1F3vjvDnRM_RjRoW5QsnOdocX8YbjsBCCqkBqhqhbcmF67AVMkz7fDPqb4iAKnxeGgS4tGAIIuuWGoOgp6Rsne3abz1bsdcdzw7wYXXJ_zr_jIaIn1mpqA-u-9onHeQlJFmY-12tANbsGc_EYgwCplDBeyNbCf8ccIw |
| Alternate Webpage(s) | https://doi.org/10.1093/oxfordjournals.pcp.a029209 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |