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Down-Regulating Receptor Interacting Protein Kinase 1 (RIP1) Promotes Oxaliplatin-Induced Tca8113 Cell Apoptosis
| Content Provider | Semantic Scholar |
|---|---|
| Author | Shan, Baozhong Ma, Feng Wang, Mingguo |
| Copyright Year | 2015 |
| Abstract | BACKGROUND Oxaliplatin is a crucial chemotherapy drug that plays an important role in colorectal cancer and oral cancer treatment. However, the molecular mechanism of oxaliplatin in killing tongue squamous cell cancer cells is still unknown. This paper investigates the mechanism of by which oxaliplatin regulates tongue squamous cell carcinoma Tca8113 cell survival and death. MATERIAL AND METHODS Tca8113 was treated with 1 μmol/L oxaliplatin for 24 h. Tca8113 cell proliferation and apoptosis were determined by MTT method and flow cytometry, respectively. Western blot was applied to detect receptor-interacting protein kinase 1 (RIP1) level. Tca8113 was transfected with siRNA RIP1 and then treated with 1 μmol/L oxaliplatin, and the cell apoptosis was detected. RESULTS We found that 1 μmol/L oxaliplatin could inhibit Tca8113 cell growth (cell survival rate was 19.3%), reduce mitochondrial membrane potential (reduce 82.3%) and phosphatidylserine eversion (positive rate was 62.7%), and activate caspase-3 (increased 2.6 times). We also found that 1 μmol/L oxaliplatin treatment could increase RIP1 expression in Tca8113 cells. Cell apoptosis rate increased after siRNA RIP1 and 1 μmol/L oxaliplatin treatment (apoptosis rate was 90.2%). CONCLUSIONS Down-regulating RIP1 promotes oxaliplatin induced Tca8113 cells apoptosis. |
| Starting Page | 3089 |
| Ending Page | 3094 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 26460489v1 |
| Volume Number | 21 |
| Journal | Medical science monitor : international medical journal of experimental and clinical research |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Apoptosis Cell Proliferation Cell Survival Cessation of life Colorectal Carcinoma Flow Cytometry Leukemia, B-Cell Lip and Oral Cavity Carcinoma Membrane Potentials Mitochondrial Membranes Phosphatidylserines Protein Kinases RIPK1 gene Squamous Epithelial Cells Squamous cell carcinoma of the tongue Western Blot Western Blotting caspase-3 cell growth monooxyethylene trimethylolpropane tristearate oxaliplatin receptor |
| Content Type | Text |
| Resource Type | Article |
