NDLI: Enhanced Resonance Light Scattering of Antibody Covalently Conjugated Gold Nanoparticles Due to Antigen-Antibody Interaction Induced Aggregation

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Enhanced Resonance Light Scattering of Antibody Covalently Conjugated Gold Nanoparticles Due to Antigen-Antibody Interaction Induced Aggregation

Content Provider	Semantic Scholar
Author	Weng, Guojun Li, Jianjun Zhao, Rusong
Copyright Year	2014
Abstract	Antibody coated nanoparticles have been used extensively in optical immunoassay for detecting various proteins. However, in most cases, the strategies for preparing antibody attached nanoparticles were based on physical adsorption, which is adverse to promote the detection limit and sensitivity of optical immunoassay. In this paper, antibody was covalently conjugated to gold nanospheres using a long chain alkanethiol: 16-mercaptohexadecanoic acid (16-MHDA). 16-MHDA modified gold nanoparticles were more stable and easier to conjugate antibody. Characterized results confirmed that antibody was covalently coated on the surface of gold nanoparticles. Owing to antigen-antibody interaction, the fabricated gold nanoparticles/antibody bioconjugates produced specific aggregation in the presence of antigen, which enhanced the resonance light scattering intensity. The enhanced intensity was highly sensitive to the concentration of antigen. The results suggested that such bioconjugates had great potential in detecting proteins using resonance light scattering with high sensitivity.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://gr.xjtu.edu.cn/c/document_library/get_file?folderId=1775454&name=DLFE-23568.pdf
Language	English
Access Restriction	Open
Subject Keyword	16-mercaptohexadecanoic acid Immunoenzyme Procedure Immunostimulating conjugate (antigen) Nanospheres Preparation Resonance Sensor
Content Type	Text
Resource Type	Article

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