Molecular survey for foot-and-mouth disease virus in livestock in Tanzania, 2008-2013.

Content Provider	Semantic Scholar
Author	Sallu, Raphael Samwel Kasanga, Christopher Jacob Mathias, Mkama Yongolo, Mmeta G. Mpelumbe-Ngeleja, C. A. R. Mulumba, Misheck Ranga, Ezekia Wambura, Philemon Nyangi Rweyemamu, Mark Knowles, Nick J. King, Donald P.
Copyright Year	2014
Abstract	Phylogeography data are of paramount importance in studying the molecular epidemiology dynamics of foot-and-mouth disease virus (FMDV). In this study, epithelial samples and oesophageal-pharyngeal fluids were collected from 361 convalescent animals (cattle and buffaloes) in the field throughout Tanzania between 2009 and 2013. The single plex real-time RT-PCR (qRT-PCR) assay for rapid and accurate diagnosis of FMDV employing the Callahan 3DF-2, 3DF-R primers and Callahan 3DP-1 probe were used. Preparation of the samples was performed according to the OIE manual, with a Kenya O serotype obtained from the attenuated vaccine serving as a positive control and samples collected from healthy animals serving as true negatives. The results indicated that 53.49% of samples (n = 176) were positive for FMDV genome by qRT-PCR, with Ct values ranging from 14 to 32. In addition, molecular typing of the FMDV genome positive samples using serotype specific primers revealed the existence of several serotypes: serotype South Africa Territory 1 (SAT1) (34.25%, n = 60), serotype A (68.92%, n = 98), serotype O (59.20%, n = 98) and SAT2 (54.54%, n = 96). The virus protein 1 sequences analysis for 35 samples was performed and the collective results indicated: 54.28% serotype O, 25.71% serotype A, 14.28% serotype SAT1 and 2.85% serotype SAT2. Therefore in this study, both the phylogenetic trees and spatial distribution of serotypes elucidated the phylodynamics of multiple FMDV field strains in Tanzania and neighbouring countries.
Starting Page	86
Ending Page	89
Page Count	4
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.ojvr.org/index.php/ojvr/article/download/736/1054
Alternate Webpage(s)	http://www.scielo.org.za/pdf/ojvr/v81n2/23.pdf
PubMed reference number	25005022v1
Alternate Webpage(s)	https://doi.org/10.4102/ojvr.v81i2.736
DOI	10.4102/ojvr.v81i2.736
Journal	The Onderstepoort journal of veterinary research
Volume Number	81
Issue Number	2
Language	English
Access Restriction	Open
Subject Keyword	Buffaloes Foot-and-Mouth Disease Virus Liquid substance Livestock Molecular Epidemiology Molecular Typing Mouth Diseases Pharyngeal structure Phylogeography Reverse Transcriptase Polymerase Chain Reaction Serotype Trees (plant) Vaccines, Attenuated Viral Proteins
Content Type	Text
Resource Type	Article