NDLI: Comparative genomics for the identification of virulence factors in Burkholderia cepacia

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Comparative genomics for the identification of virulence factors in Burkholderia cepacia

Content Provider	Semantic Scholar
Author	Seth-Smith, Helena M. B. Dennis, Alison Mahenthiralingam, Eshwar Parkhill, Julian
Copyright Year	2001
Abstract	Introduction Strains of Burkholderia cepacia are significant human pathogens, particularly causing infectious disease in patients with cystic fibrosis (CF). To date, the pathogenesis of B. cepacia infection is not well understood and few virulence factors have been identified. Nine genomovars of B. cepacia have been identified, each with distinct patterns of virulence and transmissibility. Genomovar III strains are the most predominant clinical species, being highly transmissible, highly virulent and associated with a poor prognosis in CF patients. Strains of genomovar II, also known as Burkholderia multivorans, are the second most prevalent in CF, whereas those of genomovar VII (B. ambifaria) are rarely encountered in clinical situations and are thought to be significantly less virulent. To complement the data being gathered through sequencing the genome of a genomovar III strain, and to identify regions associated with pathogenesis, a comparative genomic technique is proposed, in order to investigate differences between the genomes of B. cepacia from genomovars II, III and VII. Differential genomic DNA hybridisation1 will be performed to isolate DNA which is uniquely present or absent in each strain. The prevalence of these regions within further clinical isolates will be investigated, and the regions characterised using either the genome sequence data or direct sequence analysis. The results of this may lead to the further investigation of putative virulence factors by site directed mutagenesis and infection model testing. In this way the information provided by the genome sequencing project can be employed and novel B. cepacia virulence genes can be rapidly investigated. Example of results Results obtained in ongoing project comparing strains of Campylobacter jejuni
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.seth-smith.org.uk/images/helena/posters/hinxton2003.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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