NDLI: FITC-Dextran Fluorescein isothiocyanate dextran

Content Provider	Semantic Scholar
Copyright Year	2011
Abstract	Excitation is best performed at 493nm and uorescence measure at 518nm. Since the charge status of the uorescein moiety is dependent on the pH and ionic strength of the medium, the uorescence intensity will also vary with these parameters. The maximal intensity is observed at pH>8. Measurements in biological media will signi cantly a ect the uorescence intensity which may be enhanced or depressed. Spectral data The stability of FITC-dextran has been investigated in various media and at various temperatures. From these studies, it is concluded that the stability of FITC-dextrans in vitro and in vivo is excellent. Only at elevated pH(>9) and elevated temperatures is there a risk for hydrolysis of the uorescein label. Studies at 37 C in rabbit plasma, muscle homogenate, liver homogenate and urine established that FITC-dextrans are stable for at least 3 days. No changes in the mol.wt. and no release of uorescein moieties was noted. Further FITC-dextran was stable in 6% trichloracetic acid at room temperature for 3 days. The hydrolysis reaction shows speci c catalysis by hydroxide ions in the pH range 10-10.75 .
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://tdbcons.com/images/pdf/fitcdextran2.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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