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Analysis of nuclear protein complexes comprising α-actinin-4 by 2D-electrophoresis and mass spectrometry
| Content Provider | Semantic Scholar |
|---|---|
| Author | Khotin, Michael G. Turoverova, Lidia Podolskaya, Ekaterina P. Krasnov, I. A. Solovyeva, A. V. Aksenova, Vasilisa Pinaev, George P. Tentler, Dmitri |
| Copyright Year | 2009 |
| Abstract | Actin-binding protein α-actinin-4 is a member of the spectrin superfamily; it is located in both the cytoplasm and nucleus. However, nuclear functions of α-actinin-4 are still not clear. In this study, we analyzed the composition of nuclear protein complexes associated with α-actinin-4 in A431 cells. Using 2D electrophoresis, we found that about 50 different proteins may be associated with nuclear α-actinin-4. Major proteins of these complexes were analyzed by mass spectrometry and the following proteins have been identified: β-actin, α- and β-tubulins, ribonucleoprotein A2/B1 (regulates splicing and is associated with β-actin), peroxiredoxin-1 (involved in oxidative stress), and glycolytic enzyme D-3-phosphoglycerate dehydrogenase. The detection of these proteins in nuclear complexes with α-actinin-4 may suggest that α-actinin-4 is implicated in transcription and splicing. The presence of β-actin in these complexes was confirmed by tandem mass spectrometry (MALDI-TOF-TOF). The immunoprecipitation of nuclear proteins with antibodies against α-tubulin proved the association of α-actinin-4 with α-tubulin in the protein complex. Nuclear α-actinin-4 consists of a 105-kDa, full-size isoform and two truncated isoforms of 65 and 75 kDa; only truncated isoforms have been found in nuclear complexes with α-tubulin. These data suggest that α-actinin-4 is associated with a number of various nuclear protein complexes that may be able to carry out different functions in the cell nucleus. |
| Starting Page | 431 |
| Ending Page | 437 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| DOI | 10.1134/S1990519X09050058 |
| PubMed reference number | 19799353 |
| Journal | Medline |
| Volume Number | 3 |
| Alternate Webpage(s) | https://bio.spbu.ru/staff/pdf/09.pdf |
| Alternate Webpage(s) | https://doi.org/10.1134/S1990519X09050058 |
| Journal | Cell and Tissue Biology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
