Oxidized phospholipids trigger atherogenic inflammation in murine arteries.

Content Provider	Semantic Scholar
Author	Furnkranz, Alexander Schober, Andreas Bochkov, Valery N. Bashtrykov, Pavel Kronke, Gerhard Kadl, Alexandra Binder, Bernd R. Weber, Christian Leitinger, Norbert
Copyright Year	2005
Abstract	OBJECTIVE Lipoprotein-derived phospholipid oxidation products have been implicated as candidate triggers of the inflammatory process in atherosclerosis. However, in vivo evidence regarding the impact of oxidized phospholipids on the artery wall thus far has been elusive. Therefore, the aim of this study was to investigate if structurally defined oxidized phospholipids induce expression of atherogenic chemokines and monocyte adhesion in intact murine arteries. METHODS AND RESULTS To model the accumulation of oxidized phospholipids in the arterial wall, oxidized 1-palmitoyl-2-arachidonoyl-sn-3-glycero-phosphorylcholine (OxPAPC) was topically applied to carotid arteries in mice using pluronic gel. Using quantitative reverse-transcriptase polymerase chain reaction (PCR) and immunohistochemistry, we show that OxPAPC induced a set of atherosclerosis-related genes, including monocyte chemotactic protein 1 (MCP-1) and keratinocyte-derived chemokine (KC), tissue factor (TF), interleukin 6 (IL-6), heme oxygenase 1 (HO-1), and early growth response 1 (EGR-1). OxPAPC-regulated chemokines were also expressed in atherosclerotic lesions of apolipoprotein E-deficient (ApoE-/-) mice. In isolated perfused carotid arteries, OxPAPC triggered rolling and firm adhesion of monocytes in a P-selectin and KC-dependent manner. CONCLUSIONS Oxidized phospholipids contribute to vascular inflammation in murine arteries in vivo, initiating atherogenic chemokine expression that leads to monocyte adhesion; therefore, they can be regarded as triggers of the inflammatory process in atherosclerosis.
Starting Page	6515
Ending Page	6518
Page Count	4
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://atvb.ahajournals.org/content/atvbaha/25/3/633.full.pdf?download=true
Alternate Webpage(s)	http://atvb.ahajournals.org/content/25/3/633.full.pdf
PubMed reference number	15591214v1
Volume Number	25
Issue Number	3
Journal	Arteriosclerosis, thrombosis, and vascular biology
Language	English
Access Restriction	Open
Subject Keyword	Apolipoprotein E Apolipoproteins Arterial system Atherosclerosis CCL2 protein, human Carotid Arteries Chemokine CCL22 EGR1 gene F3 gene HMOX1 gene Inflammation Interleukin-6 Lipoproteins Monocyte Chemoattractant Proteins P-Selectin Phospholipids Phosphorylcholine Precipitating Factors Receptors, Oxidized LDL Reverse Transcriptase Polymerase Chain Reaction Selectins eIF-2 Kinase oxidation
Content Type	Text
Resource Type	Article