A C-terminal determinant of GluR6 kainate receptor trafficking.

Content Provider	Semantic Scholar
Author	Yan, Sheng Sanders, James M. Xu, Jian Zhu, Yongling Contractor, Anis Swanson, Geoffrey T.
Copyright Year	2004
Abstract	Intracellular trafficking of ionotropic glutamate receptors is regulated predominantly by determinants in the cytoplasmic C-terminal domain of the subunit proteins. Although AMPA receptors are found at the vast majority of excitatory synapses, synaptic kainate receptors exhibit a much more restricted distribution, suggesting that specific mechanisms exist for selective trafficking of these receptor proteins. In this report, we define a critical forward trafficking motif that is necessary for surface expression of the glutamate receptor 6 (GluR6) kainate receptor as well as chimeric proteins containing only the GluR6 C-terminal domain. The trafficking determinant was identified by tracking surface expression of green fluorescent protein-tagged GluR6 receptors with confocal immunofluorescence in COS-7 cells and cultured neurons and patch-clamp electrophysiology in human embryonic kidney 293 cells. Serial truncation and alanine site mutagenesis of the GluR6 subunit C terminus localized the critical motif to a seven amino acid stretch of predominantly basic residues. Alanine mutation of the trafficking motif reduced kainate receptor current amplitudes by >90% and resulted in retention of the mutated receptors in the endoplasmic reticulum. This forward trafficking domain is the first such identified for kainate receptors.
File Format	PDF HTM / HTML
DOI	10.1523/JNEUROSCI.4985-03.2004
Alternate Webpage(s)	http://www.jneurosci.org/content/jneuro/24/3/679.full.pdf
PubMed reference number	14736854
Alternate Webpage(s)	https://doi.org/10.1523/JNEUROSCI.4985-03.2004
Journal	Medline
Volume Number	24
Issue Number	3
Journal	The Journal of neuroscience : the official journal of the Society for Neuroscience
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article