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CEACAM1, a cell-cell adhesion molecule, directly associates with annexin II in a three-dimensional model of mammary morphogenesis.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kirshner, Julia Schumann, Detlef Shively, John E. |
| Copyright Year | 2003 |
| Abstract | The epithelial cell adhesion molecule CEACAM1 (carcinoembryonic antigen cell adhesion molecule-1) is down-regulated in colon, prostate, breast, and liver cancer. Here we show that CEACAM1-4S, a splice form with four Ig-like ectodomains and a short cytoplasmic domain (14 amino acids), directly associates with annexin II, a lipid raft-associated molecule, which is also down-regulated in many cancers. Annexin II was identified using a glutathione S-transferase pull-down assay in which the cytoplasmic domain of CEACAM-4S was fused to glutathione S-transferase, the fusion protein was incubated with cell lysates, and isolated proteins were sequenced by mass spectrometry. The interaction was confirmed first by reciprocal immunoprecipitations using anti-CEACAM1 and anti-annexin II antibodies and second by confocal laser microscopy showing co-localization of CEACAM1 with annexin II in mammary epithelial cells grown in Matrigel. In addition, CEACAM1 co-localized with p11, a component of the tetrameric AIIt complex at the plasma membrane, and with annexin II in secretory vesicles. Immobilized, oriented peptides from the cytoplasmic domain of CEACAM1-4S were shown to directly associate with bovine AIIt, which is 98% homologous to human AIIt, with average KD values of about 30 nM using surface plasmon resonance, demonstrating direct binding of functionally relevant AIIt to the cytoplasmic domain of CEACAM1-4S. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.bio.purdue.edu/people/faculty/faculty_files/publications/36110_1050200875.PDF |
| PubMed reference number | 14522961v1 |
| Volume Number | 278 |
| Issue Number | 50 |
| Journal | The Journal of biological chemistry |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Amino Acids Annexin A2 Annexins Bos taurus Cell Adhesion Molecules Cell secretion Cytoplasmic Domain Epithelial Cells Glutathione S-Transferase Leukemia, B-Cell Lipid A Liver and Intrahepatic Biliary Tract Carcinoma Lymphocyte antigen CD66A Malignant Neoplasms Mammary Neoplasms Morphogenesis Plasma membrane Secretory Vesicles Spectrometry Surface Plasmon Resonance Transferase Vesicle (morphologic abnormality) incubated |
| Content Type | Text |
| Resource Type | Article |
