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Translocation of cationic polystyrene microspheres associated with actin filaments on a surface coated with myosin motors
| Content Provider | Semantic Scholar |
|---|---|
| Author | Takuya, Oyama Kuniyuki, Hatori |
| Copyright Year | 2016 |
| Abstract | Muscular actin filaments can glide on a myosin-fixed glass surface by mechanochemical energy transduction. Here, we examined whether artificial objects bound to actin filaments can be translocated actively by myosin motors. Actin molecules may have a monomeric (G-actin) or fibrous form (F-actin), depending on the ionic conditions. Each form of actin molecules was mixed with a suspension of polystyrene microspheres carrying amine groups. After myosin motors were fixed on the collodion-coated glass surface, the mixture of actin molecules and microspheres was applied in the presence of adenosine triphosphate. The motion of the microspheres was monitored under a phase-contrast optical microscope. The microspheres mixed with G-actin exhibited thermal fluctuations due to insufficient binding of G-actin. In contrast, F-actin could bind to the microspheres and they were translocated in linear manner at approximately 1.5 μm/s on the myosin-coated surface. These results demonstrate that the actin filaments bound to polystyrene microspheres are capable of translocation between the actin and |
| Starting Page | 11 |
| Ending Page | 11 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://yamagata.repo.nii.ac.jp/index.php?action=pages_view_main&active_action=repository_action_common_download&attribute_id=17&block_id=29&file_no=1&item_id=3958&item_no=1&page_id=13 |
| Volume Number | 36 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
