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Role of intrahippocampal de novo protein synthesis in the processes of long-term spatial working memory in rats
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ozawa, Takaaki Yamada, Kazuo Ichitani, Yukio |
| Copyright Year | 2009 |
| Abstract | s S189 P2-n02 Implication of AMPA receptor on the enhanced fear memory in diabetic mice Megumi Asato, Masahiro Ohsawa, Hiroki Abe, Junzo Kamei Department Pathophysiol. and Therap., Sch. of Pharm. and Pharmaceut. Sci., Hoshi University, Japan Streptozotocin-induced diabetic mouse exhibits the anxiety-related behaviors under stressful condition. The aim of the present study was to investigate the mechanisms of the amygdala-dependent fear memory in diabetic mice. We examined the possible changes in the expression of AMPA and NMDA receptors in the amygdala of diabetic mice. There were no changes in the expression of total GluR1, NR2A and NR2B subunit. Under these conditions, the expression levels of GluR1 subunit phosphorylated at Ser831 and Ser845 were increased in the amygdala of diabetic mice as compared with non-diabetic mice. The increase in the duration of freezing behavior in diabetic mice was significantly attenuated by the intra-amygdala injection of an AMPA receptor antagonist NBQX which dose did not produce any effect in non-diabetic mice. These results suggest that the amygdala-dependent fear memory may be enhanced in diabetes through the potentiated amygdala AMPA receptor function. doi:10.1016/j.neures.2009.09.1017 P2-n04 Amygdala plays important roles in acquisition and retention of delay eyeblink conditioning in mice Toshiro Sakamoto1, Sonoko Ogawa1, Shogo Endo2 1 Lab behav Neuroendocrinology, Univ of Tsukuba, Tsukuba, Japan; 2 Okinawa Inst Sci and Tech, Japan Important role of cerebellum in mouse delay eyeblink conditioning has been revealed by us and other groups. However, involvement of non-cerebellar structures for this conditioning is not elucidated well. We examined the role of the amygdala and red nucleus (RN) in the eyeblink conditioning using lateral amygdala-lesioned mice or a GABAA receptor agonist, muscimol (MSC)-injected mice into the lateral amygdala or RN. Amygdala-lesioned mice showed impairment in learned eyeblink responses (LER) for a 20-day session. MSC-injected mice were also impaired with LER but they gradually acquired LER in the absence of MSC. MSC injection into the RN completely abolished LER, however, the removal of MSC led to immediate acquisition of LER to the control level. The results indicate that (1) the lateral amygdala plays important roles in the acquisition and expression; (2) RN is essential for the expression in mouse eyeblink conditioning. Our finding suggests the roles of RN similar to other animal and the potential role of amygdala in eyeblink conditioning. doi:10.1016/j.neures.2009.09.1018 P2-n05 Can rats actively control previously acquired spatial information? Masami Kaku, Kazuo Yamada, Yukio Ichitani Dept Behav Neurosci, Univ of Tsukuba, Ibaraki, Japan Evidence from pigeon studies indicates they can exert active control over the maintenance of memories. We examined using a radial maze task whether rats can actively control memory. After rats made four correct choices in the first-half in room A, 2 h delay was interposed, and rats were placed in white or black box, which signaled whether the second-half trial in room A would (remember (R)-cued trial) or would not (forget (F)-cued trial) occur. In R-cued trials, rats were required to choose the four arms that had not been chosen in the first-half. In F-cued trials, rats were required to perform no delay-interposed radial maze task in room B. After these trainings, a probe test was conducted, where rats were required to choose the rest of arms in the second-half in room A (as in the R-cued trial) even after F-cue was presented in delay period. Rats made more errors in the probe test than in the Rcued trial. Results suggest that rats can control spatial working memory they got in the first-half according to the stimuli in the delay period, and further suggest the possibility of “directed forgetting” in rats. doi:10.1016/j.neures.2009.09.1019 P2-n06 Role of intrahippocampal de novo protein synthesis in the processes of long-term spatial working memory in rats Takaaki Ozawa, Kazuo Yamada, Yukio Ichitani Dept Behav Neurosci, Univ of Tsukuba, Tsukuba, Japan We examined the role of intrahippocampal de novo protein synthesis in the processes of long-term spatial working memory (SWM) including encoding, consolidation, retention and retrieval. After male Wistar-Imamichi rats were well-trained to perform accurately on 8-arm radial maze task in which a delay of 6 h was interposed after the fourth correct choice, we tested the effects of intrahippocampal administration of anisomycin (ANI), a protein synthesis inhibitor, in various timings within a trial. Injection of ANI (50–100 g/side) 30 min before the first half and 30 min before the second half of the trial impaired the performance after delay, while the injection immediately and 2 h after the first half did not. Further, rats did not show reentering the already visited arms within the first or second half of the trial. These results suggest that (1) intrahippocampal de novo protein synthesis is not required for shot-term SWM. (2) In long-term SWM, however, encoding/consolidation and retrieval processes, but not retention process, require intrahippocampal de novo protein synthesis. doi:10.1016/j.neures.2009.09.1020 P2-n07 Roles of NMDA and AMPA receptors in the rat ventral hippocampus in spatial working memory Kazuko Hayashi, Yukio Ichitani Graduate School of Comprehensive Human Sciences, University of Tsukuba, |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/j.neures.2009.09.1020 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/S0168010209012905 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/S0168010209012905?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/j.neures.2009.09.1020 |
| Volume Number | 65 |
| Journal | Neuroscience Research |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
