Blood Flow Restriction Enhances Post–Resistance Exercise Angiogenic Gene Expression

Content Provider	Scilit
Author	Larkin, Kelly A. Macneil, R. Gavin Dirain, Marvin Sandesara, Bhanupresad Manini, Todd M. Buford, Thomas W.
Copyright Year	2012
Description	Journal: Medicine & Science In Sports & Exercise Purpose The objective of this study is to evaluate the effects of blood flow restriction (BFR) on muscle oxygenation during low-intensity resistance exercise as well as postexercise expression of molecules related to physiological angiogenesis. Methods Using a randomized cross-over design, six apparently healthy young adults (22 ± 1 yr) performed 120 unilateral knee extensions at 40% of 1 repetition maximum with and without BFR (CNTRL). Near-infrared spectroscopy was used to measure oxygenation of the vastus lateralis during exercise. Serum and muscle expression of Post–Resistance vascular endothelial growth factor (VEGF) were determined preexercise, 4 h postexercise, and 24 h postexercise. Transcript (mRNA) expression of VEGF and other angiogenic genes was also determined. Results BFR increased muscle hemoglobin (Hb) concentrations during exercise (14.4 ± 1.6 vs. 0.9 ± 1.6, P = 0.002), driven largely by an increase in deoxygenated Hb (11.0 ± 2.5 vs. 0.5 ± 1.1, P = 0.030). BFR also increased (P < 0.05) transcript expression of VEGF, VEGF-R2, hypoxia-inducible factor 1 alpha, inducible nitric oxide synthase (NOS), and neuronal NOS. The most dramatic change in response to BFR was an increase in VEGF mRNA at 4 h postexercise (4.1 ± 0.6 vs. 0.6 ± 0.2-fold change, P = 0.028). Compared with control, transcript expression of endothelial NOS, serum VEGF, or muscle protein expression of VEGF was not altered in response to BFR (P > 0.05). Conclusion Acute BFR increases postexercise expression of mRNA related to skeletal muscle angiogenesis, plausibly in response to changes in muscle Hb concentrations.
Related Links	http://europepmc.org/articles/pmc3633075?pdf=render https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3633075/pdf
Ending Page	2083
Page Count	7
Starting Page	2077
ISSN	01959131
e-ISSN	15300315
DOI	10.1249/mss.0b013e3182625928
Journal	Medicine & Science In Sports & Exercise
Issue Number	11
Volume Number	44
Language	English
Publisher	Ovid Technologies (Wolters Kluwer Health)
Publisher Date	2012-11-01
Access Restriction	Open
Subject Keyword	Journal: Medicine & Science In Sports & Exercise Sport Sciences Skeletal Muscle Resistance Exercise
Content Type	Text
Resource Type	Article
Subject	Orthopedics and Sports Medicine Physical Therapy, Sports Therapy and Rehabilitation Sports Science