Loading...
Please wait, while we are loading the content...
Similar Documents
Efferent Vagal Nerve Stimulation Attenuates Gut Barrier Injury After Burn: Modulation of Intestinal Occludin Expression
| Content Provider | Scilit |
|---|---|
| Author | Costantini, Todd W. Bansal, Vishal Peterson, Carrie Y. Loomis, William H. Putnam, James G. Rankin, Fermin Wolf, Steven Eliceiri, Brian Baird, Andrew Coimbra, Raul |
| Copyright Year | 2010 |
| Description | Journal: The Journal of Trauma and Acute Care Surgery Introduction: Severe injury can cause intestinal permeability through decreased expression of tight junction proteins, resulting in systemic inflammation. Activation of the parasympathetic nervous system after shock through vagal nerve stimulation is known to have potent anti-inflammatory effects; however, its effects on modulating intestinal barrier function are not fully understood. We postulated that vagal nerve stimulation improves intestinal barrier integrity after severe burn through an efferent signaling pathway, and is associated with improved expression and localization of the intestinal tight junction protein occludin. Methods: Male balb/c mice underwent right cervical vagal nerve stimulation for 10 minutes immediately before 30% total body surface area, full-thickness steam burn. In a separate arm, animals underwent abdominal vagotomy at the gastroesophageal junction before vagal nerve stimulation and burn. Intestinal barrier injury was assessed by permeability to 4 kDa FITC-dextran, histology, and changes in occludin expression using immunoblotting and confocal microscopy. Results: Cervical vagal nerve stimulation decreased burn-induced intestinal permeability to FITC-dextran, returning intestinal permeability to sham levels. Vagal nerve stimulation before burn also improved gut histology and prevented burn-induced changes in occludin protein expression and localization. Abdominal vagotomy abrogated the protective effects of cervical vagal nerve stimulation before burn, resulting in gut permeability, histology, and occludin protein expression similar to burn alone. Conclusion: Vagal nerve stimulation performed before injury improves intestinal barrier integrity after severe burn through an efferent signaling pathway and is associated with improved tight junction protein expression. |
| Related Links | http://europepmc.org/articles/pmc4251593?pdf=render https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4251593/pdf |
| Ending Page | 1356 |
| Page Count | 8 |
| Starting Page | 1349 |
| ISSN | 21630755 |
| e-ISSN | 21630763 |
| DOI | 10.1097/ta.0b013e3181dccea0 |
| Journal | The Journal of Trauma and Acute Care Surgery |
| Issue Number | 6 |
| Volume Number | 68 |
| Language | English |
| Publisher | Ovid Technologies (Wolters Kluwer Health) |
| Publisher Date | 2010-06-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: The Journal of Trauma and Acute Care Surgery Burn, Tight Junction, Gut, Intestinal Barrier, Permeability, Confocal Microscopy |
| Content Type | Text |
| Resource Type | Article |
| Subject | Surgery Critical Care and Intensive Care Medicine |
