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PROSTAGLANDIN E1 ATTENUATES CYTOTOXIC MECHANISMS OF PRIMED NEUTROPHILS
| Content Provider | Scilit |
|---|---|
| Author | Tamura, Douglas Y. Moore, Ernest E. Partrick, David A. Johnson, Jeffrey L. Offner, Patrick J. Silliman, Christopher C. |
| Copyright Year | 1998 |
| Description | Journal: Shock In a recent clinical trial, liposomal prostaglandin E1 (PGE1) improved oxygenation, increased compliance, and decreased ventilator dependency in patients with adult respiratory distress syndrome (ARDS), thus renewing interest in PGE1 as a potential modulator of inflammation. The neutrophil (PMN) is believed to play a key role in the development of ARDS. Consequently, we investigated the effects of PGE1 on three components of the neutrophil inflammatory response: reactive oxygen species (ROS) generation, protease release, and surface expression of adhesion molecules. Human neutrophils were incubated with PGE1 and then primed with platelet-activating factor (PAF) and activation with N-formyl-methionyl-leucylphenylalanine (fMLP). PGE1 at a dose range of (10[-8] to 10[-5] M) attenuated primed/activated (PAF/fMLP) PMN superoxide anion generation and elastase release. In contrast, PGE1 doses > or =10[-5] M were required to attenuate PAF-stimulated neutrophil upregulation of CD11b/CD18 adhesion molecules. PGE1 also diminished the duration of the PAF-induced cytosolic calcium (Ca2+) flux. Our results suggest that plasma levels of PGE1 attained in patients with ARDS may attenuate ROS and protease neutrophil cytotoxicity but may not effectively block PMN-endothelial cell (EC) adhesion. This attenuation may occur through abrogation of the Ca2+ influx. |
| Related Links | http://pdfs.journals.lww.com/shockjournal/1998/03000/PROSTAGLANDIN_E1_ATTENUATES_CYTOTOXIC_MECHANISMS.3.pdf?token=method|ExpireAbsolute;source|Journals;ttl|1507031240220;payload|mY8D3u1TCCsNvP5E421JYK6N6XICDamxByyYpaNzk7FKjTaa1Yz22MivkHZqjGP4kdS2v0J76WGAnHACH69s21Csk0OpQi3YbjEMdSoz2UhVybFqQxA7lKwSUlA502zQZr96TQRwhVlocEp/sJ586aVbcBFlltKNKo+tbuMfL73hiPqJliudqs17cHeLcLbV/CqjlP3IO0jGHlHQtJWcICDdAyGJMnpi6RlbEJaRheGeh5z5uvqz3FLHgPKVXJzdpGRzmTA0M/Vx2WsqiQOgtXKgZr89JreLQhQ7XuI25rzc5avc5c7/lN6Tu9GJ6y0z;hash|F6dTwUbA5nsr6nzLWve3QA== |
| Ending Page | 176 |
| Page Count | 6 |
| Starting Page | 171 |
| ISSN | 15400514 |
| DOI | 10.1097/00024382-199803000-00003 |
| Journal | Shock |
| Issue Number | 3 |
| Volume Number | 9 |
| Language | English |
| Publisher | Ovid Technologies (Wolters Kluwer Health) |
| Publisher Date | 1998-03-01 |
| Access Restriction | Open |
| Subject Keyword | Peripheral Vascular Disease |
| Content Type | Text |
| Resource Type | Article |
| Subject | Emergency Medicine Critical Care and Intensive Care Medicine |
