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Tandem Duplication PCR
| Content Provider | Scilit |
|---|---|
| Author | Lin, Ming-Tseh Tseng, Li-Hui Beierl, Katie Hsieh, Antony Thiess, Michele Chase, Nadine Stafford, Amanda Levis, Mark J. Eshleman, James R. Gocke, Christopher D. |
| Copyright Year | 2013 |
| Description | Journal: Diagnostic Molecular Pathology Internal tandem duplication (ITD) mutations of the FLT3 gene have been associated with a poor prognosis in acute myeloid leukemia. Detection of ITD-positive minor clones at the initial diagnosis and during the minimal residual disease stage may be essential. We previously designed a delta-PCR strategy to improve the sensitivity to 0.1% ITD-positive leukemia cells and showed that minor mutants with an allele burden of <1% can be clinically significant. In this study, we report on tandem duplication PCR (TD-PCR), a modified inverse PCR assay, and demonstrate a limit of detection of a few molecules of ITD mutants. The TD-PCR was initially designed to confirm ITD mutation of an amplicon, which was undetectable by capillary electrophoresis and was incidentally isolated by a molecular fraction collecting tool. Subsequently, TD-PCR detected ITD mutation in 2 of 77 patients previously reported as negative for ITD mutation by a standard PCR assay. TD-PCR can also potentially be applied to monitor minimal residual disease with high analytic sensitivity in a portion of ITD-positive acute myeloid leukemia patients. Further studies using TD-PCR to detect ITD mutants at diagnosis may clarify the clinical significance of those ITD mutants with extremely low allele burden. |
| Related Links | http://europepmc.org/articles/pmc3744591?pdf=render https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3744591/pdf |
| Ending Page | 155 |
| Page Count | 7 |
| Starting Page | 149 |
| ISSN | 10529551 |
| e-ISSN | 15334066 |
| DOI | 10.1097/pdm.0b013e31828308a1 |
| Journal | Diagnostic Molecular Pathology |
| Issue Number | 3 |
| Volume Number | 22 |
| Language | English |
| Publisher | Ovid Technologies (Wolters Kluwer Health) |
| Publisher Date | 2013-09-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Diagnostic Molecular Pathology Medical Laboratory Technology Internal Tandem Duplication Acute Myeloid Leukemia Minimal Residual Disease Tandem Duplication Pcr |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Molecular Biology Pathology and Forensic Medicine |
