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A simple, robust and rapid approach to detect carbapenemases in Gram-negative isolates by MALDI-TOF mass spectrometry: validation with triple quadripole tandem mass spectrometry, microarray and PCR
| Content Provider | Scilit |
|---|---|
| Author | Vogne, Christelle Prod'Hom, Guy Jaton, Katia Decosterd, Laurent Greub, Gilbert |
| Copyright Year | 2014 |
| Description | Journal: Clinical Microbiology and Infection Carbapenemases should be accurately and rapidly detected, given their possible epidemiological spread and their impact on treatment options. Here, we developed a simple, easy and rapid matrix-assisted laser desorption ionization-time of flight (MALDI-TOF)-based assay to detect carbapenemases and compared this innovative test with four other diagnostic approaches on 47 clinical isolates. Tandem mass spectrometry (MS-MS) was also used to determine accurately the amount of antibiotic present in the supernatant after 1 h of incubation and both MALDI-TOF and MS-MS approaches exhibited a 100% sensitivity and a 100% specificity. By comparison, molecular genetic techniques (Check-MDR Carba PCR and Check-MDR CT103 microarray) showed a 90.5% sensitivity and a 100% specificity, as two strains of Aeromonas were not detected because their chromosomal carbapenemase is not targeted by probes used in both kits. Altogether, this innovative MALDI-TOF-based approach that uses a stable 10-μg disk of ertapenem was highly efficient in detecting carbapenemase, with a sensitivity higher than that of PCR and microarray |
| Ending Page | O1112 |
| Starting Page | O1106 |
| ISSN | 2573508X |
| e-ISSN | 14690691 |
| DOI | 10.1111/1469-0691.12715 |
| Journal | Clinical Microbiology and Infection |
| Issue Number | 12 |
| Volume Number | 20 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2014-12-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Clinical Microbiology and Infection Medical Laboratory Technology Carbapenem Resistance Gram Negative Bacilli Maldi‐tof Ms Ms‐ms Spectrometry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Infectious Diseases Microbiology (medical) |
