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Simple Radioimmunobinding Assay for Quantitation of Sperm Antibodies of IgG Immunoglobulin Class
| Content Provider | Scilit |
|---|---|
| Author | Clarke, Gary N. |
| Copyright Year | 1988 |
| Description | Journal: American Journal of Reproductive Immunology The immunobead test (IBT) is an excellent test for initial sperm antibody screening and evaluation but is impractical when used to quantitate antibody levels using a twofold dilution series. The aim of this study, therefore, was to develop a relatively simple radioimmunobinding assay that would allow quantitation of sperm antibodies of IgG immunoglobulin class, which predominate in male sera. [125I]-Protein G was chosen as the radioligand because it binds to all IgG subclasses, but not to IgA or IgM. The results of the investigation indicate that the [125I]-protein G assay (PGA) allows efficient quantitation of sperm antibodies, as evidenced by a highly significant (P less than .0001) correlation (Spearman's, Rs = 0.94) between the PGA results and IgG-IBT titres. |
| Ending Page | 6 |
| Starting Page | 1 |
| ISSN | 2573508X |
| e-ISSN | 16000897 |
| DOI | 10.1111/j.1600-0897.1988.tb00224.x |
| Journal | American Journal of Reproductive Immunology |
| Issue Number | 1 |
| Volume Number | 18 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1988-09-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: American Journal of Reproductive Immunology Biochemical Research Immunobead Test Protein G Antisperm Antibodies |
| Content Type | Text |
| Resource Type | Article |
