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A plate assay for simultaneous screening of polysaccharide- and protein-degrading micro-organisms
| Content Provider | Scilit |
|---|---|
| Author | Ten, L. N. Im, W. -T. Kim, M. -K. Lee, S. -T. |
| Copyright Year | 2005 |
| Description | Journal: Letters in applied microbiology To develop a plate assay for simultaneous screening of polysaccharide-degrading and protein-degrading micro-organisms. A plate assay, based on the visible solubilization of small substrate particles and the formation of haloes on Petri dishes, containing a mixture of diversely coloured insoluble polysaccharides and dye-labelled collagen as chromogenic substrates, was developed. This method was successfully applied for isolating the diverse polysaccharide- and/or protein-degrading bacteria from soil and sludge samples. Selected strains were identified using 16S rDNA partial sequencing; most of them belong to the genera Bacillus, Cellulomonas and Cellulosimicrobium. This novel approach provides unique and valuable information for direct primary screening when the target of selection is micro-organisms exhibiting protein-degrading activity, polysaccharide-degrading activity or a specific combination of them. This plate assay is convenient and easy to perform, rapid, and more adaptable for screening of a large number of samples, compared with other existing methods in the literature. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2004.01637.x/pdf |
| Ending Page | 98 |
| Page Count | 7 |
| Starting Page | 92 |
| e-ISSN | 1472765X |
| DOI | 10.1111/j.1472-765x.2004.01637.x |
| Journal | Letters in applied microbiology |
| Issue Number | 2 |
| Volume Number | 40 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2005-02-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Letters in applied microbiology Analytical Chemistry Chromogenic Substrates Microbial Polysaccharases Microbial Proteases |
| Content Type | Text |
| Resource Type | Article |
