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The Purification and Properties of 2,4-Dichlorophenol Hydroxylase from a Strain of Acinetobacter Species
| Content Provider | Scilit |
|---|---|
| Author | Beadle, Carol A. Smith, Anthony R. W. |
| Copyright Year | 1982 |
| Description | Journal: Journal of Biological Inorganic Chemistry 1. 2,4-Dichlorophenol hydroxylase has been purified 13-fold from Acinetobacter grown on 2,4-dichlorophenoxyacetic acid as sole carbon source. The enzyme was estimated to be 80-90% pure by electrophoresis. 2. The enzyme has a relative molecular mass of about 240 000 and consists of four subunits of identical size. 3. The enzyme contains FAD as the prosthetic group. FAD could not be replaced by riboflavin or FMN in reconstituting active enzyme from apoenzyme. 4. The reaction catalysed is an NADPH-dependent hydroxylation of 2,4-dichlorophenol with the formation of 3,5-dichlorocatechol as product. The reaction stoichiometry is typical of a monooxygenase with an external electron donor. NADPH is the preferred reduced pyridine nucleotide substrate but the enzyme can function with NADH. 5. The enzyme possesses broad effector specificity. In addition to 2,4-dichlorophenol, 4-chlorophenol and 4-chloro-2-methylphenol are true substrates for the enzyme. A number of 'non-substrate effectors' has been found. 6. The enzyme is sensitive to thiol-inhibiting reagents. |
| Ending Page | 332 |
| Starting Page | 323 |
| ISSN | 2573508X |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1982.tb19771.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 2 |
| Volume Number | 123 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1982-04-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Biochemistry and Molecular Biology Dichlorophenol Hydroxylase Relative Molecular |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
