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Effect of troponin-tropomyosin complex and Ca2+ on conformational changes in F-actin induced by myosin subfragment-1
| Content Provider | Scilit |
|---|---|
| Author | Borovikov, Yurii S. Gusev, Nikolai B. |
| Copyright Year | 1983 |
| Description | Journal: Journal of Biological Inorganic Chemistry The interaction of regulated and unregulated actin of myosin-free ghost single fibre with myosin subfragment-1 free of 5,5′-dithiobis(2-nitrobenzoic acid) light chains was investigated by polarized microphotometry. The anisotropy of intrinsic tryptophan fluorescence of regulated actin is $Ca^{2+}$-dependent and has a maximal value at low (pCa ≥ 7) and a minimal value at high (pCa ≤ 6) concentrations of $Ca^{2+}$. The interaction of myosin subfragment-1 with actin induces cooperative changes in actin structure, which manifest themselves in a decrease in the anisotropy of tryptophan fluorescence. The cooperativity of conformational changes in actin, induced by myosin subfragment-1, is high for regulated actin in the absence of $Ca^{2+}$ and low for unregulated and regulated actin in the presence of $Ca^{2+}$. The data obtained suggest that the decrease of the flexibility of actin filaments, induced by tropomyosin or by Ca-unsaturated troponin-tropomyosin complex, results in increased cooperativity of conformational changes of actin induced by myosin subfragment-1. |
| Related Links | https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1983.tb07750.x |
| Ending Page | 369 |
| Page Count | 7 |
| Starting Page | 363 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1983.tb07750.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 2 |
| Volume Number | 136 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1983-11-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Conformational Changes Regulated Actin Actin Induced Changes in Actin Myosin Subfragment |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
