Loading...
Please wait, while we are loading the content...
Similar Documents
The heterogeneity of Campylobacter jejuni and Campylobacter coli strains isolated from healthy cattle
| Content Provider | Scilit |
|---|---|
| Author | Açik, M. N. Çetinkaya, B. |
| Copyright Year | 2005 |
| Description | Journal: Letters in applied microbiology Aims: To identify campylobacters isolated from clinically healthy cattle at species level by a multiplex polymeraseud chain reaction (m-PCR). The heterogeneity among Campylobacter jejuni and Campylobacter coli isolates was alsoud investigated by using a restriction fragment length polymorphism (RFLP) analysis of flagellin (flaA) gene.ud Methods and Results: Samples of intestinal contents, gall bladders, liver and faeces were collected from a totalud number of 1154 healthy cattle. The samples were inoculated onto Preston enrichment broth and agar. Of 1154ud samples, 301 (26Æ1%) were positive for Campylobacter spp. Using an m-PCR assay for species identification, 179ud (59Æ5%) were positive with C. jejuni specific primers while 30 (10%) were positive with C. coli specific primers.ud None of the liver samples examined was positive for C. jejuni or C. coli by mPCR. All the isolates identified asud C. jejuni and C. coli were successfully subtyped by flaA typing. Of the 209 isolates tested, 28 different flaA typesud were found. Twenty-three flaA types were identified among 179 C. jejuni isolates and the remaining five from C. coliud isolates.ud Conclusions: Although the overall results suggest that the degree of heterogeneity among the flaA genes ofud thermophilic Campylobacter strains isolated from healthy cattle is relatively high, they should be treated cautiouslyud as the number of band types for C. coli was low and band type 8 in C. jejuni was represented by a high percentageud (%58).ud Significance and Impact of the Study: The findings of the present study suggest that healthy cattle can play roleud in the contamination of environment and human food chain by Campylobacter spp |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2005.01780.x/pdf |
| Ending Page | 403 |
| Page Count | 7 |
| Starting Page | 397 |
| e-ISSN | 1472765X |
| DOI | 10.1111/j.1472-765x.2005.01780.x |
| Journal | Letters in applied microbiology |
| Issue Number | 5 |
| Volume Number | 41 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2005-11-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Letters in applied microbiology Agriculture and Animal Production Veterinary Sciences Campylobacter Jejuni Campylobacter Coli Flaa Gene Healthy Cattle Multiplex Polymerase Chain Reaction Restriction Fragment Length Polymorphism |
| Content Type | Text |
| Resource Type | Article |
