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Regional Distribution in Rat Brain of 1-Pyrroline-5-Carboxylate Dehydrogenase and Its Localization to Specific Glial Cells
| Content Provider | Scilit |
|---|---|
| Author | Thompson, S. G. Wong, P. T.-H. Leong, S. F. McGeer, E. G. |
| Copyright Year | 1985 |
| Description | Journal: Journal of Neurochemistry A possible alternative route for production of a small glutamate pool in brain is from proline or ornithine to 1-pyrroline-5-carboxylate (P5C) and thence to glutamate. The conversion from ornithine to P5C is catalyzed by ornithine delta-aminotransferase (OrnT) whereas that from proline is catalyzed by proline oxidase (PrO). The conversion of P5C to glutamate is catalyzed by 1-pyrroline-5-carboxylate dehydrogenase (PDH). Biochemical assays of PDH and PrO in various rat brain regions indicate no positive correlation between the two enzymes nor between either activity and high-affinity glutamate uptake or the regional distribution of OrnT. We have localized PDH and PrO histochemically by modifications of the Van Gelder [J. Neurochem. 12, 231-237, (1965)] method for gamma-aminobutyric acid (GABA) transaminase. The enzymes were found only in certain types of glial cells; the best stained were the Bergmann glial cells of the cerebellum but, for PDH, there was also good staining of astrocytes in the dentate area of the hippocampus. Since both these areas are believed to have heavy glutamate innervation and numerous GABA interneurons, these findings may reflect an alternative route of glutamate production in glial cells near some glutamate and/or GABA tracts but they do not support this as a possible route for glutamate formation in most brain regions. The findings do, however, provide further evidence for chemical specialization of glial cells. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.1985.tb10535.x/pdf |
| Ending Page | 1796 |
| Page Count | 6 |
| Starting Page | 1791 |
| e-ISSN | 14714159 |
| DOI | 10.1111/j.1471-4159.1985.tb10535.x |
| Journal | Journal of Neurochemistry |
| Issue Number | 6 |
| Volume Number | 45 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1985-12-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Neurochemistry Biochemistry and Molecular Biology 1‐pyrroline ‐ 5 ‐ Carboxylate 1‐pyrroline‐5‐carboxylate Dehydrogenase |
| Content Type | Text |
| Resource Type | Article |
