NDLI: Two long-chain acyl-CoA synthetases fromArabidopsis thalianainvolved in peroxisomal fatty acid β-oxidation

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Two long-chain acyl-CoA synthetases fromArabidopsis thalianainvolved in peroxisomal fatty acid β-oxidation

Content Provider	Scilit
Author	Fulda, Martin Shockey, Jay Werber, Martin Wolter, Frank P. Heinz, Ernst
Copyright Year	2002
Description	Journal: The Plant Journal Post-germinative growth of oilseeds is dependent on the breakdown of the stored lipid reserves. Long-chain acyl-CoA synthetase activities (LACS) are critically involved in this process by activating the released free fatty acids and thus feeding the β-oxidation cycle in glyoxysomes. Here we report on the identification of two LACS genes, AtLACS6 and AtLACS7 from Arabidopsis thaliana coding for peroxisomal LACS proteins. The subcellular localization was verified by co-expression studies of spectral variants of the green fluorescent protein (GFP). While AtLACS6 is targeted by a type 2 (PTS2) peroxisomal targeting sequence, for AtLACS7 a functional PTS1 as well as a PTS2 could be demonstrated. Possible explanations for this potentially redundant targeting information will be discussed. Expression studies of both genes revealed a strong induction 1 day after germination resembling the expression pattern of other genes involved in β-oxidation. Analysis of the substrate specificities of the two LACS proteins demonstrated enzymatic activity for both enzymes with the whole spectrum of fatty acids found in stored lipid reserves. These results suggest that both LACS proteins might have overlapping functions and are able to initiate β-oxidation in plant peroxisomes.
Related Links	https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-313X.2002.01405.x
e-ISSN	1365313X
DOI	10.1046/j.1365-313x.2002.01405.x
Journal	The Plant Journal
Issue Number	1
Volume Number	32
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2002-10-03
Access Restriction	Open
Subject Keyword	Journal: The Plant Journal Plant Sciences Fatty Acid Beta Oxidation
Content Type	Text
Resource Type	Article

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