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Fluorescence resonance energy transfer between sites in G-actin. The spatial relationship between Cys-10, Tyr-69, Cys-374, the high-affinity metal and the nucleotide
| Content Provider | Scilit |
|---|---|
| Author | Barden, Julian A. Remedios, Cristobal G. |
| Copyright Year | 1987 |
| Description | Journal: Journal of Biological Inorganic Chemistry Intramonomer fluorescence resonance energy transfer spectroscopy was employed to investigate the spatial relationship between labels attached to the residues Cys-10, Tyr-69, Cys-374, the high-affinity metal binding site and the nucleotide binding site in G-actin. The separation between the fluorescence donor 5-(dimethylamino)naphthalene-1-sulphonyl (Dns) chloride (dansyl chloride) used to label Tyr-69 and the acceptor 4-dimethylaminophenylazophenyl-4'-maleimide (DABM) used to label Cys-374 was found to be 3.6 nm. The distance separating Dns on Tyr-69 from DABM on Cys-10 was found to be 2.7 nm. The distance separating the acceptor DABM bound to Cys-374 from the fluorescence donor formycin A 5'-triphosphate (FTP) occupying the nucleotide binding site was determined to be 3.0 nm. A slightly larger separation was determined between the FTP site and DABM attached to Cys-10. In this case a value of 3.2 nm was obtained. The distance separating Dns on Tyr-69 from Co2+ in the high-affinity metal binding site was determined to be 1.1 nm. Finally, the separation of FTP, now acting as donor, from the Dns molecule attached to Tyr-69 and acting as the acceptor was determined to be 2.1 nm. The likely relationship between these label sites on actin is represented by a model which is used to assist in the determination of the actin structure, with particular reference to the environment of the metal and nucleotide binding sites. |
| Related Links | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1987.tb13393.x/pdf |
| Ending Page | 109 |
| Page Count | 7 |
| Starting Page | 103 |
| ISSN | 09498257 |
| e-ISSN | 14321327 |
| DOI | 10.1111/j.1432-1033.1987.tb13393.x |
| Journal | Journal of Biological Inorganic Chemistry |
| Issue Number | 1 |
| Volume Number | 168 |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 1987-10-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Biological Inorganic Chemistry Relationship Between Labels |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Inorganic Chemistry |
